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You are here:Home » Antibodies » Abs to Apoptosis, Caspase » Anti -CAD (I17) (Caspase Activated Deoxyribonuclease)

Anti -CAD (I17) (Caspase Activated Deoxyribonuclease)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Purified B
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxyribonuclease) (1,2). The human homologue of mouse CAD was more recently identified by two groups independently and termed CPAN and DFF40 (3,4). Human DFF45 and its mouse homologue ICAD are the inhibitors of CPAN/DFF40 and CAD, respectively (5). Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei (1-4). Activation of CAD/DFF40, which causes DNA degradation, is the hallmark of apoptotic cell death.
Catalog #C0106
This polyclonal antibody can be used for detection of CAD by Western blot at 1500 to 1:1000 dilution. Mouse lung tissue lysate can be used as positive control and a 40kD band should be detected.
Western blot analysis of 50 ug of murine kidney tissue lysate with anti-CAD (I17) at 1500 dilution.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
Concentration0.5mg/ml
FormSupplied as a liquid in PBS, 0.02% sodium azide.
PurityPurified IgG
ImmunogenPeptide corresponding to amino acids 205 to 222 of murine CAD (1).
SpecificityA 40kD band should be detected. Western blot analysis of 50ug of murine kidney tissue lysate with anti-CAD (I17) at 1:500 dilution.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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