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You are here:Home » Antibodies » Abs to Cadherin » Anti -Cadherin-15 (Cadherin, M, Muscle Cadherin, M-cadherin, Cadherin-14, CDH15, CDH14, CDH3)

Anti -Cadherin-15 (Cadherin, M, Muscle Cadherin, M-cadherin, Cadherin-14, CDH15, CDH14,
CDH3)

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified E B IH FC
Cadherin-15 is a 124kD type I transmembrane glycoprotein of the Cadherin superfamily of calcium-dependent homotypic adhesion molecules. Like other classical Cadherins, the 814aa human Cadherin-15 contains a signal sequence (21aa), a propeptide (29aa), an extracellular domain with five Cadherin domain repeats (ECD, 556aa), a transmembrane segment (20aa) and a cytoplasmic domain (188aa). The Cadherin repeats are responsible for cell-cell adhesion by homophilic binding on opposing cells. Intracellularly, Cadherin-15 binds beta-catenin or plakoglobin (gamma-catenin), which in turn bind alpha-catenin. Cadherin-15 also binds p120 catenin. Cadherin-15 is present during early stages of skeletal muscle development and is thought to align myoblasts for fusion. It is also present in muscle satellite cells and participates in muscle regeneration. It is expressed in the granule cell layer of the cerebellar glomerulus. Deletion of mouse Cadherin-15 has little effect in vivo, most likely due to compensation by N-Cadherin. However, Cadherin-15 upregulation and adhesion between myoblasts during induction of differentiation in vitro is required for their fusion. Human Cadherin-15 ECD shows 88% aa identity with mouse, rat, or bovine and 85% aa identity with canine Cadherin-15 ECD. Cadherin-15 is an outlier among classical Cadherins, with 40% aa identity or less in the ECD.
Catalog #C0107-02B
ApplicationsSuitable for use in ELISA, Flow Cytometry, Western Blot and Immunocytochemistry. Other applications not tested.
Recommended DilutionsFlow Cytometry: 2.5ug labels 10e6 cells from the C2C12 mouse myoblast cell line.
Western Blot: 1ug/ml detects Caderin-15 in HeLa cells.
Immunocytochemistry: 5-15ug/ml detects Cadherin-15 in immersion fixed C2C12 mouse cells.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceHuman
ConcentrationNot determined
FormSupplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant corresponding to Val22-Ala606 from human Cadherin-15, expressed in NSO cells (P55291).
SpecificityRecognizes human Cadherin-15 at ~130kD. Species Crossreactivity: mouse
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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