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C0107-10Y Rabbit Anti-Cadherin, pan

Specifications
References
Clone Type
Monoclonal
Host
Rabbit
Source
Human
Swiss Prot
P19022, P22223, P12830, P55283
Isotype
IgG
Clone Number
28E12
Grade
Purified
Applications
E WB
Crossreactivity
Hu Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C

Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development (1). The classic cadherin subfamily includes N-, P-, R-, B- and E-cadherins as well as about ten other members which are found in adherens junctions (AJ), a cellular structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with beta-catenin, gama-catenin (also called plakoglobin) and p120 catenin. beta- catenin and gamma-catenin associate with alpha-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). Unlike beta- and gamma-catenin, p120 regulates cadherin adhesive activity and trafficking rather than having a structural role in the junctional complex (1–4). E-cadherin is considered an acting suppressor of invasion and growth of many epithelial cancers (1–3). Recent studies indicate that cancer cells have up-regulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the "cadherin switch." N-Cadherin cooperates with the FGF receptor, leading to over-expression of MMP-9 and cellular invasion (3). In endothelial cells, VE-cadherin signaling, expression and localization are correlated with vascular permeability and tumor angiogenesis (5,6).

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to a conserved region of human N-, R-, E- and P-Cadherin.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Purity
Purified
Specificity
Recognizes endogenous levels of total human cadherin proteins and has a preference for N-, R-, and E-Cadherin. It does not prefer P- or VE- Cadherin. Species Crossreactivity: mouse and rat
References
(1) Wheelock, M.J. and Johnson, K.R. (2003) Annu. Rev. Cell. Dev. Biol. 19, 207–235. 2) Christofori, G. (2003) EMBO J. 22, 2318–2323. 3) Hazan, R.B. et al. (2004) Ann. NY Acad. Sci. 1014, 155–163. 4) Bryant, D.M. and Stow, J.L. (2004) Trends Cell Biol. 14, 427–434. 5) Rabascio, C. et al. (2004) Cancer Res. 64, 4373–4377. 6) Yamaoka-Tojo, M. et al. (2006) Arterioscler. Thromb.
USBio References
No references available
Conjugates
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