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Specifications

Clone Host Grade Applications Monoclonal Rabbit Supernatant E B

Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development (1). The classic cadherin subfamily includes N-, P-, R-, B- and E-cadherins as well as about ten other members which are found in adherens junctions (AJ), a cellular structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with beta-catenin, gama-catenin (also called plakoglobin) and p120 catenin. beta- catenin and gamma-catenin associate with alpha-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). Unlike beta- and gamma-catenin, p120 regulates cadherin adhesive activity and trafficking rather than having a structural role in the junctional complex (1–4). E-cadherin is considered an acting suppressor of invasion and growth of many epithelial cancers (1–3). Recent studies indicate that cancer cells have up-regulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the "cadherin switch." N-Cadherin cooperates with the FGF receptor, leading to over-expression of MMP-9 and cellular invasion (3). In endothelial cells, VE-cadherin signaling, expression and localization are correlated with vascular permeability and tumor angiogenesis (5,6).
Catalog #	C0107-10Y
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution	Western blot 1:1000
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Monoclonal
Isotype	IgG
Clone No	7H11
Host	Rabbit
Source	Human
Concentration	Not determined
Form	Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol and less than 0.02% sodium azide.
Purity	Supernatant
Immunogen	Synthetic peptide (KLH-coupled) corresponding to a conserved region of human N-, R-, E- and P-Cadherin.
Specificity	Detects endogenous levels of total cadherin proteins.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.