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You are here:Home » Antibodies » Abs to Cadherin » Anti -Cadherin E (Cadherin 1, E-Cadherin, L-CAM, Epithelial Cell Adhesion Molecule, Uvomorulin, CDH1)

Anti -Cadherin E (Cadherin 1, E-Cadherin, L-CAM, Epithelial Cell Adhesion Molecule,
Uvomorulin, CDH1)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified E B IH
In epithelia, E-Cadherin is involved in the development and maintenance of cell layers during embryogenesis and in the adult tissue. Clustering of E-Cadherin seems to occur via a, b and g catenins which link E-Cadherin to cytoskeletal actin. The E-Cadherin-catenin complex formation is crucial for cell adhesiveness. Loss of E-Cadherin expression seems to be correlated with the transformation state of carcinoma cells and with their metastatic potential.
Catalog #C0108-25J
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionImmunohistochemistry (ABC): 1:10-1:40 on frozen/paraffin sections. (Paraffin sections require microwave pretreatment.) Dilute immediately before use with PBS or TBS.
Incubation Time: 1hour at RT/ overnight at 4°C.
Optimal dilutions to be determined by the researcher.
Positive ControlHuman small intestine, MCF-7
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No5F133
HostMouse
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in 0.09% sodium azide.
PurityPurified
ImmunogenHuman uvomorulin.
SpecificityRecognizes the 120 and 80kD human ARC-1 protein in Western Blot. Species Crossreactivity: mouse, rat, and rabbit.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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