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Anti -Cadherin E (Cadherin 1, E-Cadherin, L-CAM, Epithelial Cell Adhesion Molecule, Uvomorulin, CDH1)
Anti -Cadherin E (Cadherin 1, E-Cadherin, L-CAM, Epithelial Cell Adhesion Molecule,
Uvomorulin, CDH1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Purified |
E B IH |
|
| In epithelia, E-Cadherin is involved in the development and maintenance of cell layers during embryogenesis and in the adult tissue. Clustering of E-Cadherin seems to occur via a, b and g catenins which link E-Cadherin to cytoskeletal actin. The E-Cadherin-catenin complex formation is crucial for cell adhesiveness. Loss of E-Cadherin expression seems to be correlated with the transformation state of carcinoma cells and with their metastatic potential. | | | Catalog # | C0108-25J | | Applications | Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications have not been tested. | | Recommended Dilution | Immunohistochemistry (ABC): 1:10-1:40 on frozen/paraffin sections. (Paraffin sections require microwave pretreatment.) Dilute immediately before use with PBS or TBS. | | Incubation Time: 1hour at RT/ overnight at 4°C. | | Optimal dilutions to be determined by the researcher. | | Positive Control | Human small intestine, MCF-7 | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 5F133 | | Host | Mouse | | Source | Human | | Concentration | ~0.1mg/ml | | Form | Supplied as a liquid in 0.09% sodium azide. | | Purity | Purified | | Immunogen | Human uvomorulin. | | Specificity | Recognizes the 120 and 80kD human ARC-1 protein in Western Blot. Species Crossreactivity: mouse, rat, and rabbit. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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