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You are here:Home » Antibodies » Abs to Cadherin » Anti -Cadherin E (Cadherin 1, E-Cadherin, L-CAM, Epithelial Cell Adhesion Molecule, Uvomorulin, CDH1)

Anti -Cadherin E (Cadherin 1, E-Cadherin, L-CAM, Epithelial Cell Adhesion Molecule,
Uvomorulin, CDH1)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rat Highly Purified B IP IH FC IF
Cadherins are a multifunctional family of Ca2+-dependent, transmembrane glycoproteins which promote and maintain cell adhesion in virtually all multicellular organisms. The cadherin superfamily comprises over forty proteins which are, on average, 50-60% homologous. Cadherin expression is required for the assembly of cells into solid tissues and importantly, cadherins are expressed in a tissue specific fashion. Homotypic cellular interactions are promoted by homophillic interactions between the extracellular regions of like cadherin molecules on neighboring cells. Recent crystal structure analysis of an extracellular cadherin domain suggests that individual cadherin molecules cooperate to form a linear cell adhesion zipper. In adherens junctions, cadherins are anchored to the actin cytoskeleton by interaction with the small cytoplasmic proteins b-catenin and -catenin which both bind to the actin binding protein a-catenin.bThe interaction of b- catenin with the cytoplasmic tail of cadherins and other cytoplasmic proteins, including Tcf-family transcription factors and the tumor suppressor protein APC, is thought to be mediated through a region of the b-catenin molecule containing multiple repeats of the 42 amino acid armadillo sequence motif. In addition to playing important roles in differentiation and tissue morphogenesis, cadherins also appear to play a significant role in modulating tumor invasion and metastasis. The expression of E-cadherin correlates inversely with the motile and invasive behavior of tumor cells.
Applications:
Suitable for use in Immunofluorescence, Flow Cytometry, Immunohistochemistry, Immunoblotting, Western Blot and Immunoprecipitation. Other applications not tested.
Catalog #C0108-34B
Recommended DilutionsImmunohistochemistry (Paraffin, frozen):10ug/ml for paraffin sections
Western Blot: 1-10ug/ml
Immunoblot: 1-2ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No3G44
HostRat
SourceMouse
Concentration~2mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 1% BSA. Reconstitute with 50ul sterile ddH20. Recommended Diluent: PBS, 0.1% sodium azide, 1% BSA.
PurityPuri ed by ammonium sulfate precipitation and anion exchange chromatography.
ImmunogenMouse liver E-cadherin fragment.
SpecificityRecognizes mouse Cadherin E. Moderate crossreactivity and limited af nity with human E-cadherin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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