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C0108-34B Rat Anti-Cadherin E (Cadherin 1, E-Cadherin, L-CAM, Epithelial Cell Adhesion Molecule, Uvomorulin, CDH1)

Specifications
References
Clone Type
Monoclonal
Host
Rat
Source
Mouse
Swiss Prot
P12830
Isotype
IgG2a
Clone Number
3G44
Grade
Highly Purified
Applications
FC IF IHC IP WB
Crossreactivity
Ca Hu Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C

Cadherins are a multifunctional family of Ca2+-dependent, transmembrane glycoproteins which promote and maintain cell adhesion in virtually all multicellular organisms. The cadherin superfamily comprises over forty proteins which are, on average, 50-60% homologous. Cadherin expression is required for the assembly of cells into solid tissues and importantly, cadherins are expressed in a tissue specific fashion. Homotypic cellular interactions are promoted by homophillic interactions between the extracellular regions of like cadherin molecules on neighboring cells. Recent crystal structure analysis of an extracellular cadherin domain suggests that individual cadherin molecules cooperate to form a linear cell adhesion zipper. In adherens junctions, cadherins are anchored to the actin cytoskeleton by interaction with the small cytoplasmic proteins b-catenin and γ-catenin which both bind to the actin binding protein a-catenin.bThe interaction of b- catenin with the cytoplasmic tail of cadherins and other cytoplasmic proteins, including Tcf-family transcription factors and the tumor suppressor protein APC, is thought to be mediated through a region of the b-catenin molecule containing multiple repeats of the 42 amino acid armadillo sequence motif. In addition to playing important roles in differentiation and tissue morphogenesis, cadherins also appear to play a significant role in modulating tumor invasion and metastasis. The expression of E-cadherin correlates inversely with the motile and invasive behavior of tumor cells.

Applications
Suitable for use in Immunofluorescence, Flow Cytometry, Immunohistochemistry, Immunoblotting, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilutions
Immunohistochemistry (Paraffin, frozen):10ug/ml for paraffin sections Western Blot (Reducing, non-reducing): 10ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Mouse liver E-cadherin fragment.
Form
Supplied as a lyophilized powder from 20mM TBS, pH: 7.5,|1% BSA. Reconstitute with 50ul sterile ddH20. Recommended Diluent: 20mM TBS, 10mM Calcium chloride, 1% BSA. 0.1% sodium azide.
Purity
Purified by Ion exchange chromatography.
Specificity
Recognizes mouse Cadherin E. Does not inhibit E-cadherin-dependent cell-cell contact. Species Crossreactivity: human and canine. No crossreactivity with bovine or chicken E-cadherin.
References
1. Ozawa, M., et al., EMBO J. 8: 1711-1717 (1989). 2. Takeichi, M., Science 251: 1451-1455 (1991). 3. Tang, A., et al., Nature 361: 82-85 (1993). 4. Tsarfaty, I., Science 263: 98-101 (1994). 5. Horiguchi, Y., et al., J. Histochem. Cytochem. 42: 1333-1340 (1994). 6. Aberle, H., et al., (1994) J. Cell Sci. 107: 3655-3663. 7. Roh, J-Y. and Stanley, J.R., J. Cell Biol. 128: 939-947 (1995). 8. Fannon, A., et al., J Cell Biol 129: 189-202 (1995). 9. Shapiro, L., et al., (1995) Nature 374: 327-337. 10. Babich, M. and Foti, L., Life Sciences 54: 201-208 (1995). 11. Shibamoto, S., et al., J. Cell Biol. 128: 949-957 (1995). 12. Mbalaviele, G., et al., Cancer Res 56: 4063-4070 (1996). 13. Marrs, J.A., and Nelson, W.J., Intl. Rev. of Cytology 165: 159-205 (1996). 14. Jiang, W.G., British Journal of Surgery 83: 437-446 (1996). 15. Huber, A.H., et al., Cell 90: 871-882 (1997). 16. Schofield, K., et al., Cancer 81: 293-298 (1997). 17. Soler, A.P., et al., Am. J. Pathol. 151: 471-478 (1997). 18. Dunn, R.J., et al., J. Histochem. Cytochem. 45: 129-141 (1997). 19. Kuroda, et al., Science 181: 932-835 (1998). 20. Gallicano, G.I., et al., J. Cell Biol. 143: 2009-2022 (1998). 21. Perl, A., et al., Nature Medicine 5: 286-291 (1999).
USBio References
No references available
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