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C0108-34D Rat Anti-Cadherin E (Cadherin 1, E-Cadherin, L-CAM, Epithelial Cell Adhesion Molecule, Uvomorulin, CDH1)

Specifications
References
Clone Type
Monoclonal
Host
Rat
Source
Mouse
Swiss Prot
P12830
Isotype
IgG2b
Clone Number
3G46 (ECCD-1)
Grade
Highly Purified
Applications
N
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C

Cadherins are a multifunctional family of Ca 2+ -dependent, transmembrane glycoproteins, which promote and maintain cell adhesion in virtually all multicellular organisms. The cadherin superfamily comprises over forty proteins which are, on average, 50–60% homologous (reviewed in ref 1). Cadherin expression is required for the assembly of cells into solid tissues and importantly, cadherins are expressed in a tissue specific fashion. (2) Homotypic cellular interactions are promoted by homophillic interactions between the extracellular regions of like cadherin molecules on neighboring cells. Recent crystal structure analysis of an extracellular cadherin domain suggests that individual cadherin molecules cooperate to form a linear cell adhesion zipper. (3) In adherens junctions, cadherins are anchored to the actin cytoskeleton by interaction with the small cytoplasmic proteins b-catenin and g-catenin which both bind to the actin binding protein a-catenin.

Applications
Suitable for use in Inhibition of E-Cadherin dependent cell-cell contact and epitope mapping (10). Other applications have not been tested.
Recommended Dilution
Inhibition of E-Cadherin dependent cell-cell contact: 200ug/ml for adhesion blockage. Optimal dilutions to be determined by researcher.
Hybridoma
Myeloma cell line P3-X63-Ag8
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Mouse teratocarcinoma cell line F9.
Form
Supplied as a lyophilized powder from PBS, 1% BSA. Reconstitute with 50ul sterile ddH2O.
Purity
Purified by ammonium sulfate precipitation and anion exchange chromatography.
Specificity
Recognizes mouse epithelial cadherin (E-cadherin/uvomorulin).
References
1. Marrs J. A., and Nelson W. J. (1996) International Review of Cytology 165:159-205. 2 Takeichi M.(1991) Science 251:1451-1455. 3. Shapiro L., et al and Hendrickson, W. A. (1995) Nature 374, 327-337. 4. Ozawa M, Baribault H. and Kemier R. (1989) EMBO J. 8:1711-1717. 5. Aberle H., et al, and Hoschuetzky H. (1994) J. Cell Sci. 107:3655-3663. 6. Huber A. H., Nelson W. J., and Weis W. I. (1997) Cell 90:871-882. 7. Jiang W. G. (1996) British Journal of Surgery 83:437-446. 8. Schofield K., D’Aquila T and Rimm D. L. (1997) Cancer 81:293-298 9. Soler A. P., et al, and Keshgegian A. A. (1997) American Journal of Pathology 151:471-478. 10. Nose, A. et al; Cell 61:147-155 (1990). 11. Roh, J-Y &Stanley, J.R.; Journal of Cell Biology 128 939-947 (1995). 12. Babich, M. and Foti, L.; Life Sciences 54:201-208 (1994).
USBio References
No references available
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