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C0114-02 Rabbit Anti-Calcineurin A, pan (Protein Phosphatase 2B, PP2B, CnA)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
FC IC IF IP WB
Crossreactivity
Hu Mk Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C

Calcineurin, also known as protein phosphatase 2B (PP2B), is a calmodulin-dependent, calcium-activated, serine/threonine protein phosphatase composed of a catalytic subunit (calcineurin A) and a tightly bound regulatory subunit (calcineurin B). Calcineurin A is highly homologous to protein phosphatases 1 and 2A. Calcineurin B, like calmodulin, contains four EF-hand, calcium-binding motifs.Calcineurin signaling has been implicated in a broad spectrum of cellular processes including cell-cycle regulation, stress response and apoptosis and is required for proper cardiovascular and skeletal muscle development. Calcineurin-mediated dephosphorylation of the nuclear factor of activated T cells (NFAT) transcription factor is essential for NFAT activation and nuclear translocation and early gene expression in T lymphocytes. Calcineurin is the target of the immunosuppressive drugs Cyclosporin A and FK506, both of which block the activation of quiescent T cells after T cell receptor engagement. Cyclosporin A and FK506 bind to the immunophilins, cyclophilin and FKBP respectively and the immunophilin-drug complex binds to calcineurin and blocks substrate binding.

Applications
Suitable for use in Western Blot, Immunoprecipitation, Flow Cytometry, Immunofluorescence and Immunocytochemistry. Other applications have not been tested.
Recommended Dilutions
Western Blot: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (IC): 1:50 Flow Cytometry: 1:25 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to the C-terminus of human Calcineurin A (alpha isoform). Species Sequence Homology: chicken, bovine, Xenopus and porcine, 100%
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol
Purity
Purified by Protein A and peptide affinity chromatography
Specificity
Recognizes endogenous levels of human Calcineurin A (alpha isoform). It may also recognize the beta and gamma isoforms of Calcineurin A. Does not cross-react with protein phosphatase 1 or 2A. Species Crossreactivity: mouse, rat, monkey
References
1.Rusnak, F. and Mertz, P. (2000) Physiol. Rev. 80, 1483-521.Kahl, C.R. and Means, A.R. (2003) Endocr. Rev. 24, 719-36.Schulz, R.A. and Yutzey, K.E. (2004) Dev. Biol. 266, 1-16.
USBio References
No references available
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