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You are here:Home » Antibodies » Abs to Enzymes, Phosphatase » Anti -Calcineurin A, pan (Protein Phosphatase 2B,PP2B, CnA)

Anti -Calcineurin A, pan (Protein Phosphatase 2B,PP2B, CnA)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E IP FC IF
Calcineurin, also known as protein phosphatase 2B (PP2B), is a calmodulin-dependent, calcium-activated, serine/threonine protein phosphatase composed of a catalytic subunit (calcineurin A) and a tightly bound regulatory subunit (calcineurin B) (1). Calcineurin A is highly homologous to protein phosphatases 1 and 2A. Calcineurin B, like calmodulin, contains four EF-hand, calcium-binding motifs.Calcineurin signaling has been implicated in a broad spectrum of cellular processes including cell-cycle regulation, stress response and apoptosis and is required for proper cardiovascular and skeletal muscle development (2,3). Calcineurin-mediated dephosphorylation of the nuclear factor of activated T cells (NFAT) transcription factor is essential for NFAT activation and nuclear translocation and early gene expression in T lymphocytes (2,3). Calcineurin is the target of the immunosuppressive drugs Cyclosporin A and FK506, both of which block the activation of quiescent T cells after T cell receptor engagement (2,3). Cyclosporin A and FK506 bind to the immunophilins, cyclophilin and FKBP respectively and the immunophilin-drug complex binds to calcineurin and blocks substrate binding.
Catalog #C0114-02
ApplicationsSuitable for use in Western Blot, Immunoprecipitation, Flow cytometry and Immunofluorescence. Other applications have not been tested.
Recommended Dilutions
Western Blot: 1:1000
Immunoprecipitation:1:50
Immunofluorescence (IF-IC): 1:50
Flow Cytometry: 1:25
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol, 0.02% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide (KLH-coupled) corresponding to the carboxy-terminus of human Calcineurin A (alpha isoform).
SpecificityRecognizes endogenous levels of Calcineurin A (alpha isoform). It may also recognize the beta and gamma isoforms of Calcineurin A. Does not cross-react with protein phosphatase 1 or 2A. Species Crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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