Anti -Calcineurin A, pan (Protein Phosphatase 2B,PP2B, CnA)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
E IP FC IF |
|
| Calcineurin, also known as protein phosphatase 2B (PP2B), is a calmodulin-dependent, calcium-activated, serine/threonine protein phosphatase composed of a catalytic subunit (calcineurin A) and a tightly bound regulatory subunit (calcineurin B) (1). Calcineurin A is highly homologous to protein phosphatases 1 and 2A. Calcineurin B, like calmodulin, contains four EF-hand, calcium-binding motifs.Calcineurin signaling has been implicated in a broad spectrum of cellular processes including cell-cycle regulation, stress response and apoptosis and is required for proper cardiovascular and skeletal muscle development (2,3). Calcineurin-mediated dephosphorylation of the nuclear factor of activated T cells (NFAT) transcription factor is essential for NFAT activation and nuclear translocation and early gene expression in T lymphocytes (2,3). Calcineurin is the target of the immunosuppressive drugs Cyclosporin A and FK506, both of which block the activation of quiescent T cells after T cell receptor engagement (2,3). Cyclosporin A and FK506 bind to the immunophilins, cyclophilin and FKBP respectively and the immunophilin-drug complex binds to calcineurin and blocks substrate binding. | | | Catalog # | C0114-02 | | Applications | Suitable for use in Western Blot, Immunoprecipitation, Flow cytometry and Immunofluorescence. Other applications have not been tested. | | Recommended Dilutions | | | Western Blot: 1:1000 | | Immunoprecipitation:1:50 | | Immunofluorescence (IF-IC): 1:50 | | Flow Cytometry: 1:25 | | Optimal dilutions to be determined by the researcher. | | | | Storage and Stability: | | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | Not determined | | Form | Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol, 0.02% sodium azide. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Synthetic peptide (KLH-coupled) corresponding to the carboxy-terminus of human Calcineurin A (alpha isoform). | | Specificity | Recognizes endogenous levels of Calcineurin A (alpha isoform). It may also recognize the beta and gamma isoforms of Calcineurin A. Does not cross-react with protein phosphatase 1 or 2A. Species Crossreactivity | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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