Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Enzymes, Phosphatase » Anti -Calcineurin A (CnA; Protein Phosphatase 2B, A subunit)

Anti -Calcineurin A (CnA; Protein Phosphatase 2B, A subunit)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP
Calcineurin is a Ca2+/calmodulin-dependent serine/threonine protein phosphatase. The active enzyme is a heterodimer consisting of a catalytic A subunit of 61kD and a myristylated regulatory B subunit of 19kD that binds calcium (1). The catalytic A subunit consists of an amino terminal catalytic domain, followed by calmodulin and B subunit binding sites, and a carboxyl-terminal autoinhibitory domain. Calcineurin is the target of immunosuppressant drugs such as cyclosporin A and FK506 (2). Calcineurin controls the translocation of nuclear factors of activated T cells (NFAT), a family of transcriptional activators that control the expression of cytokine genes essential to immune response, from the cytosol into the nucleus of activated T cells (3). Calcineurin may be involved in an inherited heart disease called hypertrophic cardiomyopathy (HCM), as administration of calcineurin inhibitor prevents the onset of disease in mice genetically predisposed to develop HCM (4).
Catalog #C0114-09
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications have not been tested.
Recommended DilutionWestern Blot (ECL): 0.5ug/ml
Immunoprecipitation: 10ug/ml
Optimal dilutions to be determined by researcher.
Positive ControlsRat Brain Tissue Extract
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in BBS, pH 8.4 25mM sodium borate, 100mM boric acid, 75mM sodium chloride, 5mM EDTA (disodium salt).
PurityPurified by immunoaffinity chromatography.
ImmunogenA 20 residue synthetic peptide EDFGNEKSQEHFSHNTVRGC based on the human calcineurin A (residues 264-283) was synthesized and the peptide coupled to KLH. This sequence is identical to rat and mouse calcineurin A over these residues.
SpecificityRecognizes a n~61kD protein, corresponding to the apparent molecular mass of recombinant calcineurin A. Species Crossreactivity: Human, mouse, rat, rabbit, canine, Drosophila, hamster, bovine, chicken, monkey, porcine and sheep.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links