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You are here:Home » Antibodies » Abs to Caldesmon » Anti -Caldesmon (Ser789), phosphorylated

Anti -Caldesmon (Ser789), phosphorylated

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Catalog #C1033
ApplicationsSuitable for use in Kinase assay and Western Blot. Other applications have not been tested.
Recommended DilutionKinase Assay: 0.5-2ug/ml. Active MAP Kinase 2/Erk2 (Catalog #M2362-06) was used to phosphorylate serine 789 of purified porcine (hog) stomach Caldesmon.
Western Blot: 2ug/ml. Detects phosphorlyated isoform of Caldesmon in a RIPA lysate of EGF stimulated human A431 cells. Pretreating blots with 40U/ml of lambda phosphatase prior to incubation abolished the detection of Caldesmon.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.3mg/ml
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic corresponding to amino acids 782-793 (C-QSVDKVT[ pS]PTKV) of human Caldesmon, KLH-conjugated. The immunizing sequence is identical in the rat.
SpecificityRecognizes human Caldesmon phosphorylated at Ser789 at ~Mr 140kD along with several lower molecular weight isoforms. Species Crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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