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Anti -Caldesmon (Ser789), phosphorylated
Anti -Caldesmon (Ser789), phosphorylated
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B |
|
| Catalog # | C1033 | | Applications | Suitable for use in Kinase assay and Western Blot. Other applications have not been tested. | | Recommended Dilution | Kinase Assay: 0.5-2ug/ml. Active MAP Kinase 2/Erk2 (Catalog #M2362-06) was used to phosphorylate serine 789 of purified porcine (hog) stomach Caldesmon. | | Western Blot: 2ug/ml. Detects phosphorlyated isoform of Caldesmon in a RIPA lysate of EGF stimulated human A431 cells. Pretreating blots with 40U/ml of lambda phosphatase prior to incubation abolished the detection of Caldesmon. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | ~0.3mg/ml | | Form | Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Synthetic corresponding to amino acids 782-793 (C-QSVDKVT[ pS]PTKV) of human Caldesmon, KLH-conjugated. The immunizing sequence is identical in the rat. | | Specificity | Recognizes human Caldesmon phosphorylated at Ser789 at ~Mr 140kD along with several lower molecular weight isoforms. Species Crossreactivity | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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