| CaMKII is an important member of calcium/calmodulinactivated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1, 2). CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kD (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site (3-7). The binding of Ca2+/calmodulin to its regulatory domain releases its auto inhibitory effect and activates the kinase (8). This kinase activation results in autophosphorylation at threonine 286 (8). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation (9). Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities (10-12). |
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| Catalog # | C1035-04E |
| Applications | Suitable for use in Immunofluorescence, Immunoprecipitation, Western Blot, Immunohistochemistry, RIA and ELISA. Other applications not tested. |
| Recommended Dilution | Western Blot: 0.1ug/ml. Detected CaMKII in 20ug of rat brain tissue extract by colorimetric immunoblot analysis using goat anti-mouse IgG:AP as the secondary antibody. |
| Optimal dilutions to be determined by the researcher. |
| *Recommended Secondary Antibodies: |
| I1904-04D IgG, H&L (AP) Pab GtxMo |
| I1904-06D IgG, H&L, X-Adsorbed (AP) Pab GtxMo |
| I1904-06J IgG, H&L, X-Adsorbed (AP) Pab GtxMo |
| I1904-25D IgG, H&L (AP) Pab GtxMo |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 7L1 |
| Host | Mouse |
| Source | Rat |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4, 0.09% azide, 50% glycerol. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Partially purified rat CaMKII. |
| Specificity | Recognizes ~50-60kD protein, corresponding to the apparent molecular mass of rat CaMKII alpha subunit on SDS-PAGE immunblots. Both the phosphorylated and nonphosphorylated forms are recognized. Species Crossreactivity: Mouse and bovine. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
