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C1035-25F1 Rabbit Anti-Calmodulin-Dependent Protein Kinase II, alpha, phosphorylated (Thr286) (CAMKII)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C

Calcium/calmodulin-dependent protein kinase II a (CaMKIIa) is a 50kD member of the CaMKII family of serine-threonine kinases that transduce Ca2+ signals to several target proteins, including ion channels and transcription activators. CaMKII is predominantly expressed in two isoforms in the brain: a and b. CaMKII plays an important role in neuronal plasticity and memory formation, and exerts both calcium-calmodulin-dependent and -independent activities. Autophosphorylation of CaMKIIa on threonine 286 allows the kinase to switch from a calmodulin-dependent to a calmodulin-independent state, and is required for various cellular functions including hippocampal long-term potentiation (LTP), special learning, and hippocampus-dependent memory.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic phosphopeptide derived from a region of human CaMKIIa contains threonine 286. Species sequence homology: mouse and rat.
Form
Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes Rat CaMKIIa when phosphorylated at Thr286. Species sequence homology: human and mouse (100%).
References
Weeber, E.J., et al. (2003) J. Neurosci. 23(7): 2634-2644. Beauman, S.R., et al. (2003) Cell Signal. 15(11): 1049-1057. Chin, D. and A.R. Means (2002) Biochemistry 41(47): 14001-14009. Huang, C.C., et al. (2001). J. Biol. Chem. 276(51): 48108-48117. Kim, S.A., et al. (2001) Biochem. Biophys. Res. Commun. 282(3): 773-780. Bennecib, M., et al. (2001) FEBS Lett. 490(1-2): 15-22. Giese, K.P., et al. (1998) Science 279(5352): 870-873. Cho, Y.H., et al. (1998) Science 279(5352): 867-869.
USBio References
No references available
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