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You are here:Home » Antibodies » Abs to Neuroscience » Anti -Calprotectin (MRP-8-MRP-14 Complex, S100A8-S100A9 Complex)

Anti -Calprotectin (MRP-8-MRP-14 Complex, S100A8-S100A9 Complex)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP IH FC IF
The calcium binding, migration inhibitory factor-related proteins, MRP-8 (S100A8) and MRP-14 (S100A9) belong to the S100 protein family. The expression of these proteins is largely confined to the cytosol of neutrophils and monocytes. The complex formation of these proteins is a calcium-dependent process. The S100A8/A9 heterocomplex, also called MRP-8/MRP-14 complex or calprotectin, comprises 60% of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes and is also found in monocytes, macrophages and ileal tissue eosinophils. Peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly. The S100A8/A9 complex has antibacterial, antifungal and immunomodulating and antiproliferative effects. Besides this it is a potent chemotactic factor for neutrophils. Plasma concentrations are elevated in diseases associated with increased neutrophil activity. Granulocytes terminate their existence after transmigration through the intestinal wall. Therefore calprotectin is also detectable in feces.
Catalog #C1035-61
ApplicationsSuitable for use in ELISA, Western Blot, Immunoprecipitation, Immunofluorescence, Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:50. ~22kD band observed under non-reducing conditions; antibody does not recognize the single proteins S100A8 and S100A9
Flow Cytometry: 1:50. Extracellular expression on monocytes. HL-60, platelets and lymphocytes may be used as negative control.
Immunohistochemistry (Frozen sections): 1:50. Recommended: acetone fixation; 0.1% hdyrogen peroxide treatment to reduce endogenous peroxidase activity. Inflammatory tissue may be used as positive control; normal human skin, lung or colon tissues may be used as negative control.
Immunohistochemistry (paraffin sections): 1:50.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No6A488
HostMouse
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenHuman blood monocytes
SpecificityRecognizes an epitope on the S100A8/A9 heterocomplex that is not exposed on the individual subunits S100A8 (MRP-8) or S100A9 (MRP-14). Can be used for early detection of inflammatory macrophages, for the characterization of tumorous tissues and the monitoring of peripheral blood cell cultures. Does not react with lymphocytes or platelets. Species Crossreactivity: Shows crossreaction with a subpopulation of macrophages of rhesus monkey. Does not crossreact with mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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