Carbonic anhydrase II (CA2) is a cytosolic enzyme and has the highest activity among all known CAs. Mutations in the CA2 gene result in CA II deficiency syndrome, an autosomal recessive disorder that produces osteopetrosis, renal tubular acidosis and cerebral calcification. The amino acid sequence of human CA2 is 99%, 88%, 81%, 71%, and 50% identical to that of chimpanzee, canine, mouse/rat, chicken, and fruit fly.
Applications
Suitable for use in Western Blot and ELISA. Other applications not tested.
Recommended Dilution
Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher.
Hybridoma
A fusion of mouse myeloma with B cells obtained from a rat immunized with purified, E. coli derived, recombinant human CA2.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to Ser2-Lys260 of human CA2 expressed in E. coli.
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human carbonic anhydrase II. No cross-reactivity with rhCA-1, -3, -4, -5A, -5B, -6, -7, -8, or -9.