Apoptosis is related to many diseases and development. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. CARD containing proteins include some caspases, Apaf-1, CARD4, IAPs, RICK, ARC, RAIDD, Bcl-10, and ASC. A novel CARD- containing protein was recently identified and designated CARD10 (1). This protein belongs to the membrane- associated guanylate kinase-like (MAGUK) family of proteins that can function as molecular scaffolds that assist assembly of signal transduction molecules (2). CARD 10 interacts with Bcl-10, a Bcl protein that promotes apoptosis, caspase-9 maturation and activation of NF-κB (1,3). CARD 10 and the related protein CARMA1 also associate with NEMO, the regulatory subunit of the IκK complex (4), demonstrating its importance in the regulation of NF-κB transcription factor activation

Suitable for use in Western Blot. Other applications not tested.

Western Blot: 5-10ug/ml Optimal dilutions to be determined by the researcher.

EL4 cell lysate

May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Synthetic peptide CARD10 14aa at the C- terminus of human CARD10 (Genbank accession No. AAK26165).

Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.|

Purified by immunoaffinity chromatography.|

Recognizes human CARD10. Species Crossreactivity: mouse