Cardiotin is a recently discovered, high molecular weight protein complex (300kD) located in the mitochondrial membrane. The cardiotin structure exists of subunits of 60kD and 100kD. Both subunits contain the same amino-terminal 14 amino-acid sequence, showing high homology to human skeletal muscle a-actinin, suggesting that the tetrameric configuration of the cardiotin protein structure is a transmembrane complex with the N-terminus at the cytoplasmic side of the membrane, able to interact with actin.
During cardiac contractile dysfunction, the cardiotin distribution is affected in pathlogical cardiomyocytes i.e. chronic ischemic myocardium. R2G and SR-2, SR-3 and SR-4, monoclonal cardiotin antibodies can be used in immunohistochemistry for the detection of a disturbed mitochondrial activity in cardiomyocytes i.e. during chronic ischemia or chronic atrial fibrillation.
Applications
Suitable for use in Immunohistochemistry (human frozen sections) and Western Blotting (swine). Other applications not tested.
Optimal dilutions to be determined by researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. No stabilizing proteins added.
Purity
Purified by Protein G affinity chromatography.
Specificity
Reacts exclusively with the 300kD cardiotin protein complex in immunoblotting assays. The antibody stains cardiomyocytes and epithelial cells and tissues. Species crossreactivity: Human, porcine.
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