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You are here:Home » Antibodies » Abs to Catenin » Anti -Catenin, beta, phosphorylated (Ser675)

Anti -Catenin, beta, phosphorylated (Ser675)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IP IH
Catenin-ß plays a central role in cell adhesion, bridging between cadherins and actin cytoskeleton. These activities are regulated through its differential interaction with several molecular partners, including cell-cell adherens junction (AJ) molecules, components of its degradation complex, and transcription factors (1, 2, 3). Beta-Catenin is composed of three domains: a central armadillo repeat domain and two N- and C-terminal tails. Beta-catenin can be phosphorylated by serine-threonine kinase, such as GSK-3 (4). The phosphorylated beta-catenin affects the protein association of beta-catenin to E-cadherin and alpha-cateinin that occurs in the cell. This process can be reversed by dephosphorylation of beta-catenin (5).
Catalog #C2069-47J
ApplicationsSuitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 0.5-2ug/ml detects a band ~88kD in treated mouse embryonic cell lysate and HEK293 whole cell lysate.
Immunohistochemistry: 1-2ug/ml
Immunoprecipitation: 3-5ug/extract from 10e6 cells
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
PurityPurified by epitope affinity chromatography.
ImmunogenSynthetic peptide (KRLpSVEL) of C-terminal portion of human Beta-catenin at the phosphorylated site of Serine 675.
SpecificityRecognizes human Catenin-Beta at phosphorylation site of Ser675 Species Crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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