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Anti -Catenin, beta, phosphorylated (Ser675)
Anti -Catenin, beta, phosphorylated (Ser675)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
E B IP IH |
|
| Catenin-ß plays a central role in cell adhesion, bridging between cadherins and actin cytoskeleton. These activities are regulated through its differential interaction with several molecular partners, including cell-cell adherens junction (AJ) molecules, components of its degradation complex, and transcription factors (1, 2, 3). Beta-Catenin is composed of three domains: a central armadillo repeat domain and two N- and C-terminal tails. Beta-catenin can be phosphorylated by serine-threonine kinase, such as GSK-3 (4). The phosphorylated beta-catenin affects the protein association of beta-catenin to E-cadherin and alpha-cateinin that occurs in the cell. This process can be reversed by dephosphorylation of beta-catenin (5). | | | Catalog # | C2069-47J | | Applications | Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Western Blot: 0.5-2ug/ml detects a band ~88kD in treated mouse embryonic cell lysate and HEK293 whole cell lysate. | | Immunohistochemistry: 1-2ug/ml | | Immunoprecipitation: 3-5ug/extract from 10e6 cells | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | ~0.2mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide. | | Purity | Purified by epitope affinity chromatography. | | Immunogen | Synthetic peptide (KRLpSVEL) of C-terminal portion of human Beta-catenin at the phosphorylated site of Serine 675. | | Specificity | Recognizes human Catenin-Beta at phosphorylation site of Ser675 Species Crossreactivity | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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