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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IP IH IF

Adherens junctions (AJ; also referred to as zonula adherens) are required for both the establishment and maintenance of epithelial layers (1). In addition, these junctions have been identi ed in several other cell types including cardiac myocytes and broblasts (1). AJ subserve several important functions including: mediating intercellular adhesion, sensing the presence of neighboring cells, and anchoring the actin cytoskeleton (1,10). AJ are multiprotein complexes that are assembled around cell adhesion molecules called cadherins. Cadherins are a multifunctional family of Ca2+-dependent, transmembrane glycoproteins which promote cell-cell adhesion (6). The cadherin extracellular domain mediates homophilic interactions between like cadherin molecules on neighboring cells, while the intracellular domain interacts with several cytoplasmic proteins which include: a-catenin, b-catenin, g-catenin (plakoglobin), and the tyrosine kinase substrate p120cas (2,4,7,9,13,16). Cadherin-catenin interactions are required for complete cadherin activity and regulate the interaction between cadherins and the actin-based cytoskeleton (2,4,6,9,13,17). In fact, deletion of the cadherin cytoplasmic domain produces an adhesion-defective molecule that is unable to interact with the cytoskeleton (6,8).
Catalog #	C2069-50C
Applications	Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilution	Immuno uorescence: 5–10ug/ml
	Western Blot: 1ug/ml
	Immunoprecipitation: 2–5ug
	Immunohistochemistry (Formalin-fixed and paraffin-embedded tissue): 2–10ug/ml. Requires heat induced epitope retrieval.
	Optimal dilutions to be determined by the researcher.
Positive Control	HeLa, A431 and WI-38 human cell lines
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	3G60
Host	Mouse
Source	Chicken
Concentration	0.5mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Fusion protein consisting of the maltose binding protein fused to a 100 amino acid segment of the C-terminus of chicken b-Catenin.
Specificity	Recognizes chicken b-Catenin protein (~92kD). Does not crossreact with the related 83kD g-catenin (plakoglobin) protein. Also speci cally detects an additional 120kD protein of unknown identity in extracts derived from A431 cells. This band is not detected in lysates derived from HeLa cells or human broblasts. Species Crossreactivity: Human & mouse. Reactivity with rat is likely but not con rmed.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.