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C2069-50C Mouse Anti-Catenin, beta

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Chicken
Isotype
IgG1,k
Clone Number
3G60
Grade
Affinity Purified
Applications
IF IHC IP WB
Crossreactivity
Ch Hu Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C

Adherens junctions (AJ; also referred to as zonula adherens) are required for both the establishment and maintenance of epithelial layers (1). In addition, these junctions have been identified in several other cell types including cardiac myocytes and fibroblasts (1). AJ subserve several important functions including: mediating intercellular adhesion, sensing the presence of neighboring cells, and anchoring the actin cytoskeleton (1,10). AJ are multiprotein complexes that are assembled around cell adhesion molecules called cadherins. Cadherins are a multifunctional family of Ca2+-dependent, transmembrane glycoproteins which promote cell-cell adhesion (6). The cadherin extracellular domain mediates homophilic interactions between like cadherin molecules on neighboring cells, while the intracellular domain interacts with several cytoplasmic proteins which include: a-catenin, b-catenin, g-catenin (plakoglobin), and the tyrosine kinase substrate p120cas (2,4,7,9,13,16). Cadherin-catenin interactions are required for complete cadherin activity and regulate the interaction between cadherins and the actin-based cytoskeleton (2,4,6,9,13,17). In fact, deletion of the cadherin cytoplasmic domain produces an adhesion-defective molecule that is unable to interact with the cytoskeleton (6,8).

Applications
Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunofluorescence: 5–10ug/ml Western Blot: 1ug/ml Immunoprecipitation: 2–5ug Immunohistochemistry (Formalin-fixed and paraffin-embedded tissue): 2–10ug/ml. Requires heat induced epitope retrieval. Optimal dilutions to be determined by the researcher.
Positive Control
HeLa, A431 and WI-38 human cell lines
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Fusion protein consisting of the maltose binding protein fused to a 100 amino acid segment of the C-terminus of chicken b-Catenin.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes chicken b-Catenin protein (~92kD). Does not crossreact with the related 83kD g-catenin (plakoglobin) protein. Also specifically detects an additional 120kD protein of unknown identity in extracts derived from A431 cells. This band is not detected in lysates derived from HeLa cells or human fibroblasts. Species Crossreactivity: Human & mouse. Reactivity with rat is likely but not confirmed.
References
1. Geiger, B., et al., Curr. Opin. Cell Biol. 1: 103–109 (1989). 2. Ozawa, M., et al., PNAS USA 87: 4246–4250 (1990). 3. Peifer, M., et al., Cell 63: 1167–1178 (1990). 4. McCrea, P.D., et al., J. Biol. Chem. 266: 4514–4520 (1991). 5. McCrea, P.D., et al., Science 25: 1359–1361 (1991). 6. Takeichi, M., et al., Science 51: 1451–1455 (1991). 7. Knudsen, K.A., et al., J. Cell. Biol. 18: 671–679 (1992). 8. Ozawa, M., et al., J. Cell. Biol. 116: 989–996 (1992). 9. Johnson, K.R., et al., Exp. Cell Res. 207: 252–260 (1993). 10. Peifer, M., et al., Science 262: 1667–1668 (1993). 11. Rubinfeld, B., et al., Science 262: 1731–1734 (1993). 12. Su, L.-K., et al., Science 262: 1734–1737 (1993). 13. Hinck, L., et al., J. Cell. Biol. 125: 1327–1340 (1994). 14. Kowalczk, A.P., et al., J. Biol. Chem. 269: 31,214–31,223 (1994). 15. Peifer, M., et al., Cell 76: 789–791 (1994). 16. Daniel, J.M., et al., Mol. Cell. Biol. 270: 4819–4824 (1995). 17. Knudsen, K.A., et al., J. Biol. Chem. 130: 67–77 (1995). 18. Bornslaeger, E.A., et al., J. Cell Biol. 134: 985–1001 (1996). 19. Yamaoto, M., et al., J. Biol. Chem. 272(19): 12,492–12,494 (1997). 20. Sakanaka, C., et al., Pro. Natl. Acad. Sci. 95: 3020–3023 (1998). 21. Wu, S., et al., PNAS USA 95(25): 14,979–14,984 (1998). 22. Tesco, G., et al., J. Biol. Chem. 273(51): 33,909–33,914 (1998). 23. Li, Y., et al., Mol. Cell. Biol. 18: 7216–7224 (1998).
USBio References
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