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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IP

Catalog #	C2069-50D
Applications	Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution	ELISA: 0.1-1.0ug/ml
	Western Blot after IP: 1-2ug/ml. Crossreactivity with a ~100kD protein of unknown identity is sometimes observed.
	Immunoprecipitation: 5-10ug. Speci cally immunoprecipitate the ~ 92kD b-Catenin protein from native cell lysates.
	Optimal dilutions to be determined by the researcher.
Positive Controls	HeLa and A431 lysates.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	~0.25mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Purity	Purified by immunoaffinity chromatography.
Immunogen	A 15 amino acid synthetic peptide derived from the C-terminus of the human and mouse b-catenin protein.
Specificity	Recognizes human b-catenin. Species Crossreactivity: chicken, mouse, rat and Xenopus..
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.