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Specifications

Clone Host Grade Applications Monoclonal Mouse E B IP

The catenins are polypeptides that bind to the conserved cytoplasmic tail of cadherins and are required for cadherin function (2). a-catenin is a 102kD protein that is associated with the cadherin indirectly through its interaction with b-catenin and g-catenin (3). b-Catenin and its homolog plakoglobin associate directly with the cadherin (4). Thus, the cell-cell adherens junction is a structure composed of the transmembrane cadherin, which is associated directly with either b-catenin or plakoglobin, which in turn assoctiates directly with a-catenin. a- catenin then mediates the interaction between the cadherin-catenin complex and the actin cytoskeleton (5,6). a-catenin has been characterized as a tumor suppressor, suggesting that it may have a regulatory function in regulation to its structural role (7).
Catalog #	C2069-52B
	b-Catenin links cadherin cell adhesion molecules to the cytoskeleton and has recently been implicated in the wnt/wingless signaling pathway and in the progression of neoplasia. b-Catenin associates with the tumor suppressor adenomatous polypopsis coli (APC) and functions as a co-activator of the TCF transcription factor family. It is regulated by glycogen synthase kinase (GSK) 3b, which phosphorylates and targets b-Catenin for ubiquitin-mediated proteolysis. Stabilization of b-catenin, either by wnt-mediated inhibition of GSK3b or by mutation (as found in numerous colorectal tumors and melanoma cell lines), results in activation of TCF responsive genes which are thought to play key roles in development and cancer progression.
Application(s)	ELISA, Immunoprecipitation, Western Blot
	Optimal conditions must be determined individually for each application.
	Positive Control included
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	6D205
Host	Mouse
Source	Human
Immunogen	Recombinant -catenin.
Specificity	Recognizes the N-terminus (exon 2) of human, mouse and rat -catenin. Detects a band of ~90kD by Western Blot.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.