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C2086-72K Rat Anti-Caspase 2 (ICH-1, Nedd2)

Specifications
References
Clone Type
Monoclonal
Host
Rat
Source
Human
Swiss Prot
P42575
Isotype
IgG2a,k
Clone Number
6D217 (11B4)
Grade
Affinity Purified
Applications
IP WB
Crossreactivity
Ca Hu Mk Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C

Caspase-2 is a Class I caspase with a long prodomain necessary for nuclear localization. Upon activation of the apoptotic pathway, the procaspase is cleaved into smaller fragments. Caspase-2 is the nuclear apoptotic respondent to cellular genotoxic stress or mitotic catastrophe and is involved in the activation cascade of caspases responsible for apoptosis execution. Activation occurs upon recruitment to a complex containing a p53-induced death domain protein, PIDD. This suggests caspase-2 can be a nuclear initiator caspase with a requirement for caspase-9 and caspase-3 activation in downstream apoptotic events. In apoptotic pathways resulting from UV-induced DNA damage, processing of caspase-2 occurs downstream of mitochondrial dysfunction and of caspase-9 and caspase-3 activation, extending a possible role for caspase-2 as a parallel effector caspase.

Applications
Suitable for use in Immunoprecipitation and Western Blot. For Flow Cytometry, Immunocytochemistry, and Immunohistochemistry see C2086-72H. Other applications not tested.
Recommended Dilutions
Western Blot: 1-2ug/ml Immunoprecipitation: 1-5ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to human caspase-2 p19 fragment.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes an epitope in the human p19 subunit of, caspase-2. Detects bands of ~51kD (procaspase-2), ~32kD and ~18kD (cleavage products) by Western Blot. Species Crossreactivity: mouse, rat, monkey and canine.
References
1.L.A. O’Reilly, et al.; Cell Death Differ. 9, 832 (2002) 2. P. Lassus, et al.; Science 297: 1352 (2002) 3. S.H. Read, et al.; J. Cell Biol. 159: 739 (2002) 4. A. Tinel and J. Tschopp; Science 304: 843 (2004) 5. A.G. Yakovlev, et al.; J. Biol. Chem. 279: 28367 (2004)
USBio References
No references available
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