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Anti -Caspase 3, Cleaved (Asp175) (CPP-32, Apoptain, Yama, SCA-1)
Anti -Caspase 3, Cleaved (Asp175) (CPP-32, Apoptain, Yama, SCA-1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B IP IH IC FC IF |
|
| Caspase 3 is one of the key executioners of apoptosis. It is responsible either partially or totally for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP). Activation of caspase 3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 subunits. Cleavage of caspase 3 requires aspartic acid at the P1 position. | | | | | | Applications: | | | Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunofluorescence/Immunocytochemistry. Other applications not tested. | | | Catalog # | C2087-16A | | Recommended Dilutions | Flow Cytometry: 1:800 | | Western Blot: 1:1000 | | Immunohistochemistry (Paraffin): 1:300 | | Immunohistochemistry (Frozen): 1:600 fixed in 3% Fromaldehyde. | | Immunofluorescence (IC): 1:400 | | Immunoprecipitation: 1:100 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | Not Determined | | Form | Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. | | Purity | Purified by Protein A and immunoaffinity chromatography. | | Immunogen | Synthetic peptide corresponding to amino-terminal residues adjacent to Asp175 of human caspase-3. Species Sequence Homology: porcine, bovine and canine | | Specificity | Recognizes cleaved human Caspase 3 (Asp175). Detects endogenous levels of the large fragment (17/19kD) of activated caspase-3 resulting from cleavage adjacent to Asp175. Does not recognize full length caspase-3 or other cleaved caspases. Detects non-specific caspase sunstrates by Western Blot. | | Species Crossreactivity | Mouse, rat and monkey. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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