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You are here:Home » Antibodies » Abs to Apoptosis, Caspase » Anti -Caspase 3 (CPP-32, Apoptain, Yama, SCA-1)

Anti -Caspase 3 (CPP-32, Apoptain, Yama, SCA-1)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH
Caspase 3 is ubiquitously expressed and like other caspases is synthesized as an inactive (32kD) proenzyme. Upon activation, Caspase 3 is cleaved at Asp28-Ser29 and Asp175-Ser176 thereby generating two subunits of 17kD and 12kD, respectively. Recent studies have implicated that Caspase 3 is associated with the induction of apoptosis. Activation of Caspase 3 occurs in response to variety of apoptotic inducers including Fas mediated apoptosis.
Catalog #C2087-22C3
ApplicationsSuitable for use in Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionsImmunoprecipitation (Native verified): 2ug/mg of protein lysate (Use Protein A)
Western Blot: 1ug/ml for 2 hours at RT
Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 minutes at RT
Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.
Positive ControlJurkat cells, Tonsil and appendix
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No3F49
HostMouse
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See C2087-22C3X.
PurityPurified by Protein A affinity chromatography.
ImmunogenRecombinant full length human Caspase 3 protein. MW of Antigen
SpecificityRecognizes human Caspase 3 (CPP32).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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