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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IH

Cathepsin B is a cysteine protease commonly overexpressed in metastisizing cancer cells. An intracellular (lysosomal) enzyme in peptidase family C1 (papain family), Cathepsin B will hydrolyze proteins with broad specificity for peptide bonds. Preferentially cleaves -Arg-Arg bonds in small molecule substrates (thus differing from cathepsin L). In addition to being an endopeptidase, shows peptidyl-dipeptidase activity, liberating C-terminal dipeptides.
Catalog #	C2097-11M
Applications	Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilutions	Immunohistochemistry: 1:200. Paraffin sections after microwave treatment.
	Optimal dilution determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	6D231
Host	Mouse
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.01% thimerosal.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Recombinant human procathepsin B.
Specificity	Recognizes the epitope E212PGYSP217 of human cathepsin B. Recognizes mature human single or two chain cathepsin B and procathepsin B. Does not crossreact with mature cathepsins H, K, L, S, V, W or procathepsins L, S, W.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.