Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IH

Cathepsin L is a cystein protease. It is important for the overall degradation of proteins in lysosomes. Cathepsin L substrates include collagen, elastin and alpha 1 protease inhibitor. The protein is initially expressed as procathepsin L (42kD) and is proteolytically processed into 30kD and 21kD forms. This antibody recognises both precursor and mature forms.
Catalog #	C2097-70F
Applications	Suitable for use in Immunohistochemistry and Western Blot . Other applications not tested.
Recommended Dilution	Western Blot: 2ug/ml
	Immunohistochemistry (formalin/acetone fixed tissues): 4ug/ml
	Optimal dilution determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Monoclonal
Isotype	IgG
Clone No	9G334
Host	Mouse
Source	Human
Concentration	As reported
Form	Supplied as a lyophilized powder from 1.2% sodium acetate, 2% BSA, 0.2% sodium azide. Reconstitute with sterile PBS to concentration of 0.1-1mg/ml.
Purity	Purified by affinity chromatography.
Immunogen	Procathepsin L isolated from human lung cancer cell line EPLC32M1. The epitope is within the sequence GYGFEST (265-271 in procathepsin L and 169-175 in the mature cathepsin L molecule).
Specificity	Recognizes human Cathepsin L. Species Crossreactivity: mouse, rat
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.