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You are here:Home » Antibodies » Abs to Cathepsin » Anti -Cathepsin L (MEP, CTSL, CTSL1)

Anti -Cathepsin L (MEP, CTSL, CTSL1)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH
Cathepsin L is a cystein protease. It is important for the overall degradation of proteins in lysosomes. Cathepsin L substrates include collagen, elastin and alpha 1 protease inhibitor. The protein is initially expressed as procathepsin L (42kD) and is proteolytically processed into 30kD and 21kD forms. This antibody recognises both precursor and mature forms.
Catalog #C2097-70F
ApplicationsSuitable for use in Immunohistochemistry and Western Blot . Other applications not tested.
Recommended DilutionWestern Blot: 2ug/ml
Immunohistochemistry (formalin/acetone fixed tissues): 4ug/ml
Optimal dilution determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone No9G334
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a lyophilized powder from 1.2% sodium acetate, 2% BSA, 0.2% sodium azide. Reconstitute with sterile PBS to concentration of 0.1-1mg/ml.
PurityPurified by affinity chromatography.
ImmunogenProcathepsin L isolated from human lung cancer cell line EPLC32M1. The epitope is within the sequence GYGFEST (265-271 in procathepsin L and 169-175 in the mature cathepsin L molecule).
SpecificityRecognizes human Cathepsin L. Species Crossreactivity: mouse, rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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