Suitable for use in Flow Cytometry. Other applications not tested.

Flow Cytometry: 1:50 Optimal dilutions to be determined by the researcher.

1. Five x 10e5 cultured HPB-MLT human tumor cells were washed and incubated 45 minutes on ice with 80ul of C252-02M at 1:50 (10ug/ml). 2. Cells were washed three times, fixed and analyzed by FACS. 3. Cells stained positive with a mean shift of 1.40 log10 fluorescent units when compared to a Mouse IgG1/R-PE negative control at a similar concentration. 4. Binding was blocked when cells were pre incubated 10 minutes with 20ul of 0.5 mg/ml C2252-02J.

May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.

Membrane antigen preparation of human thymocytes.

Supplied as a liquid in PBS, pH 7.5, 0.2% BSA, 0.04% sodium azide, 15% glycerol. Labeled with R-Phycoerythrin (PE).

Purified by Protein A affinity chromatography.

Recognizes human CD1b at ~45kD. Specificity was confirmed using CD1b transfectants.