Functional Application: Antibody will activate T cells expressing CD3e.

Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested.

Optimal dilutions to be determined by the researcher.

May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing APC conjugates will result in a substantial loss of activity. APC conjugates are sensitive to light.

Human thymocytes/Sezary T cells

Supplied as a liquid in PBS, pH 7.2, 15% glycerol, 0.2% BSA, 0.05% sodium azide. Labeled with allophycocyanin.

Purified by Protein A affinity chromatography.

Recognizes the 20kD epsilon chain of the CD3 molecule complex. Antibody will activate T cells expressing CD3e.