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You are here:Home » Antibodies » Abs to CD Markers » Anti -CD3 (Early T Cell Marker, T Cell receptor Complex, TCR)

Anti -CD3 (Early T Cell Marker, T Cell receptor Complex, TCR)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified IH FC
Catalog #C2254-38B
ApplicationsSuitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:100; Use 10ul to label 10e6 cells in 100ul.
Immunohistochemistry: Frozen sections. Does not stain formalin fixed paraffin embedded sections.
Optimal working dilutions to be determined by researcher.
HybridomaSpleen cells from immunized SD rats were fused with cells of the NSO mouse myeloma cell line.
Recommended Secondary AntibodyI1903-13C: F(ab')2 Goat anti-rat IgG: RPE (mouse adsorbed)
Recommended Negative ControlI1904-82D1: Rat IgG2a Negative Control
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No3H698
HostRat
SourceMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
PurityPurified by Protein G affinity chromatography.
SpecificityRecognizes the mouse CD3 antigen, which is expressed by mature T cells. The antibody may be used to trigger proliferation and cytotoxicity of CD3 positive cells. For optimal staining incubations should be performed at room temperature.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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