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Anti -CD3 (Early T Cell Marker, T Cell receptor Complex, TCR)
Anti -CD3 (Early T Cell Marker, T Cell receptor Complex, TCR)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rat |
Affinity Purified |
IH FC |
|
| Catalog # | C2254-38B | | Applications | Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Flow Cytometry: 1:100; Use 10ul to label 10e6 cells in 100ul. | | Immunohistochemistry: Frozen sections. Does not stain formalin fixed paraffin embedded sections. | | Optimal working dilutions to be determined by researcher. | | Hybridoma | Spleen cells from immunized SD rats were fused with cells of the NSO mouse myeloma cell line. | | Recommended Secondary Antibody | I1903-13C: F(ab')2 Goat anti-rat IgG: RPE (mouse adsorbed) | | Recommended Negative Control | I1904-82D1: Rat IgG2a Negative Control | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | Clone Type | Monoclonal | | Isotype | IgG2a | | Clone No | 3H698 | | Host | Rat | | Source | Mouse | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide. | | Purity | Purified by Protein G affinity chromatography. | | Specificity | Recognizes the mouse CD3 antigen, which is expressed by mature T cells. The antibody may be used to trigger proliferation and cytotoxicity of CD3 positive cells. For optimal staining incubations should be performed at room temperature. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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