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You are here:Home » Antibodies » Abs to CD Markers » Anti -CD4 (CD4 Receptor, CD4mut, T Cell Surface Antigen T4/Leu3, T Cell Surface Glycoprotein CD4

Anti -CD4 (CD4 Receptor, CD4mut, T Cell Surface Antigen T4/Leu3, T Cell Surface
Glycoprotein CD4

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH FC
Rat CD4 cell surface glycoprotein, a 55kD molecule expressed by helper T cells and weakly by monocytes. Monoclonal antibodies clustered as CD4 detect most thymocytes and a subpopulation of peripheral blood T cells, called T helper cells (Th). In addition, CD4 is expressed on monocytes and macrophages. The CD4 antigen is a 55kD glycoprotein which plays a role in the recognition of foreign antigens presented to T cells by MHC class II molecules. Furthermore, CD4 acts as a receptor for HIV-1 by binding the viral protein gp120.
Catalog #C2255-04D
Applications
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:100-1:200, Use 10ul to label 10e6 cells in 100ul.
Immunohistochemistry: Frozen and paraffin. Reacts with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde).
Optimal dilution determined by the researcher
Recommended Secondary ReagentsI1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1903-13A: IgG, F(abí)2, X-Adsorbed (PE) Pab: Gt xMo
I1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab Gt xMo
Recommended Negative ControlsI1904-78Y: Mouse IgG1 Negative Control
HybridomaNS1 myeloma cells with spleen cells from Balb/c mice.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG1
Clone No6A230
HostMouse
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenRat Thymocyte Membrane Glycoproteins.
SpecificityRecognizes the rat CD4 cell surface glycoprotein, 55kD. Inhibits proliferation and IL-2 production in the MLR reaction.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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