| Rat CD4 cell surface glycoprotein, a 55kD molecule expressed by helper T cells and weakly by monocytes. Monoclonal antibodies clustered as CD4 detect most thymocytes and a subpopulation of peripheral blood T cells, called T helper cells (Th). In addition, CD4 is expressed on monocytes and macrophages. The CD4 antigen is a 55kD glycoprotein which plays a role in the recognition of foreign antigens presented to T cells by MHC class II molecules. Furthermore, CD4 acts as a receptor for HIV-1 by binding the viral protein gp120. |
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| Catalog # | C2255-04D |
| Applications | |
| Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. |
| Recommended Dilution | Flow Cytometry: 1:100-1:200, Use 10ul to label 10e6 cells in 100ul. |
| Immunohistochemistry: Frozen and paraffin. Reacts with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde). |
| Optimal dilution determined by the researcher |
| Recommended Secondary Reagents | I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo |
| I1903-13A: IgG, F(ab’)2, X-Adsorbed (PE) Pab: Gt xMo |
| I1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab Gt xMo |
| Recommended Negative Controls | I1904-78Y: Mouse IgG1 Negative Control |
| Hybridoma | NS1 myeloma cells with spleen cells from Balb/c mice. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 6A230 |
| Host | Mouse |
| Source | Rat |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | Rat Thymocyte Membrane Glycoproteins. |
| Specificity | Recognizes the rat CD4 cell surface glycoprotein, 55kD. Inhibits proliferation and IL-2 production in the MLR reaction. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
