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C2275-07 Mouse Anti-CD24 (GPI-linked surface Mucin, Nectadrin)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1
Clone Number
8.B.76
Grade
Purified
Applications
FC IC IHC
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
4°C Do not freeze

CD24 is a single chain glycosylphosphatidylinositol (GPI)-anchored cell surface sialoglycoprotein. Expressed early in B lymphocytes ontogeny and lost at the plasma cell stage. In contrast, CD24 antigen is absent from T lymphocytes, NK cells, monocytes, erythrocytes and platelets.

Applications
Suitable for use in Flow Cytometry, Immunohistochemistry, Immunocytochemistry and studies of peripheral blood B cells. Other applications have not been tested.
Recommended Dilutions
Immunohistochemistry: Frozen sections Immunocytochemistry: Cell smears Optimal dilutions to be determined by the researcher.
Hybridoma
NS1/AG4.1 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
Lyophilized powder and reconstituted products must be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Reconstitute with sterile dH2O. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Human bone marrow malignant cells from a non-B, non-T acute leukemia.
Form
Supplied as a lyophilized powder from PBS, 1mg/ml BSA. Reconstitute with 1ml sterile ddH2O.
Purity
Purified from ascites
Specificity
Recognizes human CD24 at 35-45kD. Recognizes B cells from pre-B stage to mature-B stage.
References
1. Boucheix, C., et al., Leuk. Res., 9 (5):597–604 (1985) 2. Duperray, C., et al., J. Immunol, 145 (11):3678–3683 (1990) 3. Chappel, S., Kay, R., Humphries, R.K. “CD24 Workshop Panel report”, 1997, Leucocyte Typing VI, White Cell Differentiation Antigens, Kishimoto, T., et. al., Eds., Garland Publishing, Inc., 147–149.
USBio References
No references available
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