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Anti -CD42b alpha (Glycoprotein Ib alpha, GPIba)
Anti -CD42b alpha (Glycoprotein Ib alpha, GPIba)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Sheep |
Affinity Purified |
E B FC |
|
| Platelet glycoprotein Ib alpha chain (GPIb-alpha), also known as CD42b alpha, is a 145kD platelet type I transmembrane protein. It binds von Willebrand factor (VWF) and thrombin, and is part of the platelet GPIb-IX-V complex, which plays important roles in vascular biology, including hemostasis and thrombosis. GPIb-alpha is also a member of the family of proteins with leucine-rich repeats, which mediate protein-protein interactions. The 610 residue mature human GPIb-alpha contains a 489aa extracellular domain (ECD) (aa17-505) and a 100aa cytoplasmic region (aa527-626). Proteolytic cleavage of the GPIb-alpha ECD from platelet surface releases soluble GPIb-alpha (glycocalicin), which is found in plasma. The ECDs of human, mouse and canine GPIb-Alpha share approximately 50% aa sequence identity. | | | Catalog # | C2395-20 | | Applications | Suitable for use in Flow Cytometry, Western Blot and ELISA. Other applications not tested. | | Recommended Dilution | Flow Cytometry: 25ug/ml; Add 10ul of diluted antibody to 1-2.5x10e5 cells in a total reaction volume no greater than 200ul. Tested using blood-derived CD41+ platelets. | | Western Blot: 0.1-0.2ug/ml; Detection limit under non-reducing and reducing conditions of rhGPIb-alpha is ~1ng/lane. | | ELISA: 0.5-1ug/ml; Detection limit of rhGPIb-alpha is ~5ng/well. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Sheep | | Source | Human | | Concentration | ~0.2mg/ml (after reconstitution) | | Form | Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS. | | Purity | Purified by affinity chromatography. | | Immunogen | Recombinant corresponding to aa17-505 of human CD42b alpha expressed in NSO cells (Accession # P07359). | | Specificity | Recognizes human CD42b alpha (GPIb-alpha, extracellular domain). | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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