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You are here:Home » Antibodies » Abs to Carbohydrates, Glycoproteins » Anti -CD42b alpha (Glycoprotein Ib alpha, GPIba)

Anti -CD42b alpha (Glycoprotein Ib alpha, GPIba)

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified E B FC
Platelet glycoprotein Ib alpha chain (GPIb-alpha), also known as CD42b alpha, is a 145kD platelet type I transmembrane protein. It binds von Willebrand factor (VWF) and thrombin, and is part of the platelet GPIb-IX-V complex, which plays important roles in vascular biology, including hemostasis and thrombosis. GPIb-alpha is also a member of the family of proteins with leucine-rich repeats, which mediate protein-protein interactions. The 610 residue mature human GPIb-alpha contains a 489aa extracellular domain (ECD) (aa17-505) and a 100aa cytoplasmic region (aa527-626). Proteolytic cleavage of the GPIb-alpha ECD from platelet surface releases soluble GPIb-alpha (glycocalicin), which is found in plasma. The ECDs of human, mouse and canine GPIb-Alpha share approximately 50% aa sequence identity.
Catalog #C2395-20
ApplicationsSuitable for use in Flow Cytometry, Western Blot and ELISA. Other applications not tested.
Recommended DilutionFlow Cytometry: 25ug/ml; Add 10ul of diluted antibody to 1-2.5x10e5 cells in a total reaction volume no greater than 200ul. Tested using blood-derived CD41+ platelets.
Western Blot: 0.1-0.2ug/ml; Detection limit under non-reducing and reducing conditions of rhGPIb-alpha is ~1ng/lane.
ELISA: 0.5-1ug/ml; Detection limit of rhGPIb-alpha is ~5ng/well.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceHuman
Concentration~0.2mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
PurityPurified by affinity chromatography.
ImmunogenRecombinant corresponding to aa17-505 of human CD42b alpha expressed in NSO cells (Accession # P07359).
SpecificityRecognizes human CD42b alpha (GPIb-alpha, extracellular domain).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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