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C2395-20 Sheep Anti-CD42b alpha (Glycoprotein Ib alpha, GPIba)

Specifications
Clone Type
Polyclonal
Host
Sheep
Source
Human
Swiss Prot
P07359
Isotype
IgG
Grade
Affinity Purified
Applications
E FC WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

Platelet glycoprotein Ib alpha chain (GPIb-alpha), also known as CD42b alpha, is a 145kD platelet type I transmembrane protein. It binds von Willebrand factor (VWF) and thrombin, and is part of the platelet GPIb-IX-V complex, which plays important roles in vascular biology, including hemostasis and thrombosis. GPIb-alpha is also a member of the family of proteins with leucine-rich repeats, which mediate protein-protein interactions. The 610 residue mature human GPIb-alpha contains a 489aa extracellular domain (ECD) (aa17-505) and a 100aa cytoplasmic region (aa527-626). Proteolytic cleavage of the GPIb-alpha ECD from platelet surface releases soluble GPIb-alpha (glycocalicin), which is found in plasma. The ECDs of human, mouse and canine GPIb-Alpha share approximately 50% aa sequence identity.

Applications
Suitable for use in Flow Cytometry, Western Blot and ELISA. Other applications not tested.
Recommended Dilution
Flow Cytometry: 25ug/ml; Add 10ul of diluted antibody to 1-2.5x10e5 cells in a total reaction volume no greater than 200ul. Tested using blood-derived CD41+ platelets. Western Blot: 0.1-0.2ug/ml; Detection limit under non-reducing and reducing conditions of rhGPIb-alpha is ~1ng/lane. ELISA: 0.5-1ug/ml; Detection limit of rhGPIb-alpha is ~5ng/well. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to His17-Leu505 of human CD42b alpha expressed in NSO cells.
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Purity
Purified by affinity chromatography.
Specificity
Recognizes human CD42b alpha (GPIb-alpha, extracellular domain).
Conjugates
Pricing
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