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C2399-09B Mouse Anti-CD45 (PTPRC, Receptor-type Tyrosine-protein Phosphatase C, Leukocyte Common Antigen, L-CA, T200)

Specifications
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1,k
Clone Number
SPM496
Grade
Affinity Purified
Applications
IHC
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

CD45 (PTPRC) is a family of high-molecular weight glycoproteins uniquely expressed on the surface of all leukocytes and their hematopoietic progenitors. The CD45 family consists of multiple members that are all products of a single gene. Structurally, CD45 is composed of one fibronectin extracellular domain, one transmembrane domain, and two cytoplasmic tyrosine phosphatase domains. It is essential for B- and T-cell activation. For both cell-types, CD45 has been shown to interact with kinases including Lyn, src family kinase Lck, and Janus-family kinases. In T cells, CD45 is required for the initiation of receptor signaling by dephosphorylating a negative regulatory tyrosine in the C-terminal tail of Lck. CD45 may also negatively regulate Lck by dephosphorylating the tyrosine in the activation loop, thereby attenuating Lck activity.

Applications
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunohistochemistry (Formalin fixed paraffin embedded): 1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Stimulated human leucocytes
Form
Supplied as a liquid in 10mM PBS, pH 7.4,1% BSA buffer, 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography.|
Specificity
Recognizes human PTPRC.
Conjugates
Pricing
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