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C2399-14E Mouse Anti-CD45 (Leukocyte Common Antigen, LCA, Ly-5 T200) (PE)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Conjugate
PE
Isotype
IgG2a
Clone Number
F10-89-4
Grade
Purified
Applications
FC IHC IP WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

CD45, also known as the leukocyte common antigen (LCA), exists as four different isoforms (180, 190, 205, 220kD) that are generated by alternative splicing of three exons. LCA is found on all cells of hematopoietic origin, except erythrocytes and platelets. CD45 expression is required for T cell and B cell antigen receptor-mediated activation.1-4

Applications
• Flow cytometry • Immunohistochemistry (frozen sections) • Immunoprecipitation • Western blotting
Recommended Dilution
Flow Cytometry: 10ul/10e6 cells Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Form
Supplied as a liquid in PBS, 0.09% sodium azide and a stabilizing agent. Labeled with PE.
Purity
Purified
Specificity
Recognizes human CD45.
References
1. McMichael, A.K., P.C.L. Beverly, S. Cobbold, M.J. Crumpton, W. Gilks, F.M. Gotch, N. Hogg, M. Horton, N. Ling, I.C.M. MacLennan, D.Y. Mason, C. Milstein, D. Spiegelhalter, and H. Waldmann, eds. 1987. Leukocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, Oxford.|2. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte Antigens Facts Book, 2nd Edition, CD45 Section, Academic Press, New York, p. 244.|3. Dalchau, R., J. Kirkley, and J.W. Fabre. Eur. J. Immunol. 10:737.|4. Thomas, M.L. 1989. Annu. Rev. Immunol. 7:339.|5. Trowbridge, I.S., and M.L. Thomas. 1994. Annu. Rev. Immunol. 12:85.
USBio References
No references available
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