Suitable for use in Flow Cytometry. Other applications have not been tested.

Flow Cytometry: Neat-1:10 Optimal working dilutions to be determined by researcher.

Spleen cells from immunized DA rats were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line

I1904-84L: IgG2b Negative Control (PE)

Store product at 4°C before and after reconstitution. DO NOT FREEZE! Lyophilized and reconstituted products are stable for 12 months after receipt at 4°C. Reconstitute lyophilized powder with sterile ddH2O prior to use and store remainder as an undiluted liquid. Caution: PE conjugates are sensitive to light. For maximum recovery of product centrifuge the original vial prior to removing the cap.

Canine thymocytes.

Supplied as a lyophilized powder from PBS, 1% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE). Reconstitute with 1ml sterile ddH2O.

Purified by Protein G affinity chromatography from tissue culture supernatant.

Recognizes canine CD45. Immunoprecipitates antigen of 180/200kD from Con-A blasts (1). Expressed on all leukocytes in canine peripheral blood. Crossreactivity: all outbred mongrels and beagles tested.