Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilutions
Flow Cytometry: Neat. 10ul labels 10e6 cells or 100ul whole blood. Optimal dilutions to be determined by the researcher.
Hybridoma
P3/NS1/1-Ag4-1 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
Immunogen
Human IL-2 dependent T cells.
Form
Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes the low molecular weight isoform (180kD) of human CD45RO. The antigen is expressed by a functional subset of T cells with memory phenotype. In peripheral blood, stains 40-80% of lymphocytes and all monocytes and granulocytes.
References
1. Smith, S.H., Brown, M.H., Rowe, D., Callard, R.E., and Beverley, P.C.L. (1986). Functional subsets of human helper-inducer cells defined by a new monoclonal antibody, UCHL1. Immunology 58: 63-72.2. Norton, A.J., Ramsay, A.D., Smith, S.H
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