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You are here:Home » Antibodies » Abs to Integrin » Anti -CD49c (Integrin alpha3, Very Late Antigen3a chain, VLA3a)

Anti -CD49c (Integrin alpha3, Very Late Antigen3a chain, VLA3a)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH FC
Catalog #C2404-08C
ApplicationsSuitable for use in Flow Cytometry, Inhibition and Immunohistochemistry. Other aapplications not tested.
Recommended DilutionsInhibition: Inhibits SCC9 cell adhesion to human and EHS laminin, but not laminin peptide GD-2. Potentiates inhibition of SKOV-3 cell adhesion to EHS laminin by anti-integrin alpha 5 antibody GoH3. Does not inhibit either cellís adhesion to fibronectin or collagen type IV.
Immunohistochemistry: On acetone-fixed frozen human epithelial tissues including tongue, foreskin and ovary. Not for use on formalin-fixed tissue.
Flow cytometry: Stains human squamous cell carcinoma (SCC9), ovarian carcinoma (SKOV-3) and umbilical vein endothelial cells (HUVEC).
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No2Q994
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS pH 7.6, 0.1% sodium azide.
PurityPurified by Protein A chromatography.
ImmunogenHuman squamous cell carcinoma cells (SCC9).
SpecificityRecognizes human alpha-3 integrin (CD49c).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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