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C2404-08C Mouse Anti-CD49c (Integrin alpha3, Very Late Antigen3a chain, VLA3a)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
P26006
Isotype
IgG1,k
Clone Number
2Q994 (ASC-6)
Grade
Affinity Purified
Applications
FC IHC
Crossreactivity
Hu
Accession #
NM_002204.1, NM_005501.1
Shipping Temp
Blue Ice
Storage Temp
-20°C

Applications
Suitable for use in Flow Cytometry, Inhibition and Immunohistochemistry. Other aapplications not tested.
Recommended Dilutions
Inhibition: Inhibits SCC9 cell adhesion to human and EHS laminin, but not laminin peptide GD-2. Potentiates inhibition of SKOV-3 cell adhesion to EHS laminin by anti-integrin alpha 5 antibody GoH3. Does not inhibit either cell’s adhesion to fibronectin or collagen type IV. Immunohistochemistry: On acetone-fixed frozen human epithelial tissues including tongue, foreskin and ovary. Not for use on formalin-fixed tissue. Flow Cytometry: Stains human squamous cell carcinoma (SCC9), ovarian carcinoma (SKOV-3) and umbilical vein endothelial cells (HUVEC). Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Human squamous cell carcinoma cells (SCC9)
Form
Supplied as a liquid in PBS pH 7.6, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human alpha-3 integrin (CD49c).
References
1. Pattaramalai, S., et al. Exper. Cell Res. 222: 281–290 (1996). 2. Tissue Antigens 48:354 (1996).
USBio References
No references available
Conjugates
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