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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified IH FC IF

CD52 is a 25-29kD glycosylphosphatidylinositol (GPI)- anchored single chain, a remarkably small glycoprotein, which is typically expressed at high levels on thymocytes, lymphocytes, NK cells and monocytes/macrophages. It is expressed on some granulocytes, on epithelial cells of the male reproductive tract and on the majority of lymphoid malignancies, but not on erythrocytes and platelets. A somatic defect in the synthesis of GPI anchors (for example lymphocytes from patients with paroxysmal nocturnal haemoglobinuria (PNH)) may cause lack of expression of CD52 on the cell surface. CD52 may then be used as an index to diagnose PNH besides CD55 and CD59. All CD52 antibodies are characterized by a strong lytic activity with the human complement, irrespective of antibody isotype.
Catalog #	C2407-51
Applications	Suitable for use in Indirect Immunofluorescence, Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended Dilutions	Flow Cytometry: 0.125ug labels 100ul peripheral blood using Indirect Immunofluorescence.
	Immunohistochemistry: Acetone-fixed frozen and formalin-fixed, paraffin sections.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone Type	Monoclonal
Isotype	IgG2b
Clone No	5F44
Host	Mouse
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.0, 0.1% sodium azide.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Human tonsil
Specificity	Recognizes human CD52. Reacts with normal human peripheral blood lymphocytes (90.6%). Does not react with normal human peripheral blood granulocytes.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.