| CD54 permits antigen-independent adhesion between lymphocytes and their targets and may be used to study cell-cell interactionsor for rhinovirus and malarial studies. The LFA-1 binding site is located in domain 1 of ICAM-1. CD54 antigen is a membrane glycoprotein of ~90kD with a wide tissue distribution that includes vascular endothelium and many cells of the immune system. CD54 is weakly expressed on resting peripheral blood lymphocytes. Upon activation by mitogens, the CD54 antigen is strongly expressed on B-cells, T-cells, macrophages and granulocytes. |
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| Catalog # | C2409-14B |
| Applications | Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested. |
| Recommended Dilutions | Flow Cytometry: 1:50-1:100. Use 10ul to label 10e6 cells in 100ul. |
| Immunohistochemistry (Frozen): 1:50-1:100 |
| Optimal working dilutions to be determined by researcher. |
| Recommended Positive Control | Human Tonsil |
| Recommended Secondary Antibodies | I1904-65W: IgG, F(ab’)2 (HRP) Pab Rb xMo |
| F(ab')2 rabbit anti mouse IgG: RPE conjugate |
| Recommended Negative Control | I1904-78R: IgG1 Murine Negative Control |
| Hybridoma | Sp2/0-Ag14 myeloma cells with spleen cells from Balb/c mice. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 3H1547 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Human monocytes |
| Specificity | Recognizes human CD54, a 90kD glycoprotein. Expressed by many cells following activation by inflammatory mediators. Blocks CD54 function. Species Crossreactivity: porcine |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
