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You are here:Home » Antibodies » Antibodies-CD Markers » Anti -CD54 (ICAM-1, Intercellular Adhesion Molecule 1, Human Rhinovirus Receptor, Precursor, BB2, Major Group Rhinovirus Receptor, P3.58)

Anti -CD54 (ICAM-1, Intercellular Adhesion Molecule 1, Human Rhinovirus Receptor,
Precursor, BB2, Major Group Rhinovirus Receptor, P3.58)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IP IH FC
CD54 permits antigen-independent adhesion between lymphocytes and their targets and may be used to study cell-cell interactionsor for rhinovirus and malarial studies. The LFA-1 binding site is located in domain 1 of ICAM-1. CD54 antigen is a membrane glycoprotein of ~90kD with a wide tissue distribution that includes vascular endothelium and many cells of the immune system. CD54 is weakly expressed on resting peripheral blood lymphocytes. Upon activation by mitogens, the CD54 antigen is strongly expressed on B-cells, T-cells, macrophages and granulocytes.
Catalog #C2409-14B
ApplicationsSuitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested.
Recommended DilutionsFlow Cytometry: 1:50-1:100. Use 10ul to label 10e6 cells in 100ul.
Immunohistochemistry (Frozen): 1:50-1:100
Optimal working dilutions to be determined by researcher.
Recommended Positive ControlHuman Tonsil
Recommended Secondary AntibodiesI1904-65W: IgG, F(abí)2 (HRP) Pab Rb xMo
F(ab')2 rabbit anti mouse IgG: RPE conjugate
Recommended Negative ControlI1904-78R: IgG1 Murine Negative Control
HybridomaSp2/0-Ag14 myeloma cells with spleen cells from Balb/c mice.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
Clone No3H1547
FormSupplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenHuman monocytes
SpecificityRecognizes human CD54, a 90kD glycoprotein. Expressed by many cells following activation by inflammatory mediators. Blocks CD54 function. Species Crossreactivity: porcine
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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