USBio Logo

C2409-14B Mouse Anti-CD54 (ICAM-1, Intercellular Adhesion Molecule 1, Human Rhinovirus Receptor, Precursor, BB2, Major Group Rhinovirus Receptor, P3.58)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
P05362
Isotype
IgG1
Clone Number
3H1547 (15.2)
Grade
Affinity Purified
Applications
FC IHC IP
Crossreactivity
Hu Po
Shipping Temp
Blue Ice
Storage Temp
-20°C

CD54 permits antigen-independent adhesion between lymphocytes and their targets and may be used to study cell-cell interactionsor for rhinovirus and malarial studies. The LFA-1 binding site is located in domain 1 of ICAM-1. CD54 antigen is a membrane glycoprotein of ~90kD with a wide tissue distribution that includes vascular endothelium and many cells of the immune system. CD54 is weakly expressed on resting peripheral blood lymphocytes. Upon activation by mitogens, the CD54 antigen is strongly expressed on B-cells, T-cells, macrophages and granulocytes.

Applications
Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested.
Recommended Dilutions
Flow Cytometry: 1:50-1:100. Use 10ul to label 10e6 cells in 100ul. Immunohistochemistry (Frozen): 1:50-1:100 Immunohistochemistry: Paraffin sections Optimal working dilutions to be determined by researcher.
Recommended Positive Control
Human Tonsil
Recommended Secondary Antibodies
I1904-65W: IgG, F(ab’)2 (HRP) Pab Rb xMo F(ab')2 rabbit anti mouse IgG: RPE conjugate
Recommended Negative Control
I1904-78R: IgG1 Murine Negative Control
Hybridoma
Sp2/0-Ag14 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Human monocytes.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes human CD54, a 90kD glycoprotein. Blocks CD54 function. Species Crossreactivity: porcine
References
1. J. Cell Biol. (1992) 116: 1527-1535. 2. Cell (1992) 68: 71-81. 3. Treadwell, M.D. et al. (1996). Increased neutrophil adherence to endothelial cells exposed to asbestos. Toxicol Appl. Pharmacol. 139: 62-70.
USBio References
No references available
Conjugates
Pricing
Order
Proceed to Checkout
Cart Summary
ProductSizeListYour PriceQtyExt Price
Subtotal:Subtotal:
Subtotal:Subtotal:
Total Coupon Savings:Total Coupon Savings:()
Your cart is currently empty.
- Coupon Code
Recently Viewed
Contact Us

Visit our technical library or contact our support staff to answer your questions.

Telephone:
1-800-520-3011

Library | Contact

Distributors

For customers outside of the United States, please use one of our many distributors.

View Distributors

Payment Methods

We accept the following payment methods as well as pay-by-invoice.

MasterCard Visa PayPal
© 2023-2024 United States Biological - All Rights Reserved