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C2414-10B Mouse Anti-CD61 (Glycoprotein IIIa, Integrin beta3)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
P05106
Isotype
IgG1,k
Clone Number
SAP
Grade
Affinity Purified
Applications
IC IHC WB
Crossreactivity
Hu
Accession #
NM_000212
Shipping Temp
Blue Ice
Storage Temp
-20°C
Platelet glycoprotein IIIa, ITGB3, GP3A

Applications
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilutions
Immunocytochemistry: 5-20ug/ml showed positive immunostaining for Integrin 3 (CD61) in HuVec cells fixed with 4% paraformaldehyde followed by permeabilization with 0.25% Triton X-100. Western Blot: Detects Integrin 3 (CD61) in WM852 and 1205Lu whole cell extracts (1,2). Immunohistochemistry: Detects Integrin 3 (CD61) in malignant melanoma sections (1). Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Human platelet membrane fraction
Form
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human Integrin b3 (CD61), Mr ~115kD.
References
Application References:|1. Van Belle, P., et al., Human Path. 30: 562-567, 1999.|2. Hsu, M.-Y., et al., Am. J. Path. 156: 1515-1525, 2000.|General References:|3. Hamm, C.W., Ann. Rev. Med. 54: 425-435, 2001.|4. Kumar, C.C., Curr. Drug Targets 4: 123-131, 2003.
USBio References
No references available
Conjugates
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