Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilutions
Immunocytochemistry: 5-20ug/ml showed positive immunostaining for Integrin 3 (CD61) in HuVec cells fixed with 4% paraformaldehyde followed by permeabilization with 0.25% Triton X-100. Western Blot: Detects Integrin 3 (CD61) in WM852 and 1205Lu whole cell extracts (1,2). Immunohistochemistry: Detects Integrin 3 (CD61) in malignant melanoma sections (1). Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Human platelet membrane fraction
Form
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human Integrin b3 (CD61), Mr ~115kD.
References
Application References:|1. Van Belle, P., et al., Human Path. 30: 562-567, 1999.|2. Hsu, M.-Y., et al., Am. J. Path. 156: 1515-1525, 2000.|General References:|3. Hamm, C.W., Ann. Rev. Med. 54: 425-435, 2001.|4. Kumar, C.C., Curr. Drug Targets 4: 123-131, 2003.
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