USBio Logo

C2426-02B Mouse Anti-CD71 (Transferrin Receptor, TR, Tfr, TfnrR) (Biotin)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Conjugate
Biotin
Isotype
IgG1
Clone Number
7H180
Grade
Affinity Purified
Applications
FC
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
4°C (-20°C Glycerol)

Human CD71 is a type II transmembrane glycoprotein found on activated leukocytes. CD71 binds transferrin and mediates uptake of iron through endocytosis and recycling.

Applications
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution
Flow Cytometry: 5ug/ml Immunohistochemistry: frozen sections Optimal dilutions to be determined by the researcher.
Flow Cytometry Protocol
1. 5x10e5 cultured human HPB-MLT cells were washed and incubated for 45 minutes on ice with 80ul of C2426-02B at 5ug/ml. 2. Cells were washed twice and incubated with Streptavidin/R-Phycoerythrin (S7973-88P). 3. Cells were washed three times, fixed and analyzed by FACS. 4. Cells stained positive with a mean shift of 1.9 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. 5. Binding was 97% blocked when cells were pre-incubated with 20ul of 0.5mg/ml C2426-02A.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Human PBL
Form
Supplied as a liquid in PBS, pH 7.5, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with Biotin.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human CD71 at ~95kD.
References
1)Leukocyte Typing IV (W. Knapp, et al, eds.) Oxford University Press, Oxford, (1989) p. 455-460. |2)Leukocyte Typing VI (T. Kishimoto, et al, eds.) Garland Publishing, Inc., New York (1997) p. 524-527.|
USBio References
No references available
Conjugates
Pricing
Order
Proceed to Checkout
Cart Summary
ProductSizeListYour PriceQtyExt Price
Subtotal:Subtotal:
Subtotal:Subtotal:
Total Coupon Savings:Total Coupon Savings:()
Your cart is currently empty.
- Coupon Code
Recently Viewed
Contact Us

Visit our technical library or contact our support staff to answer your questions.

Telephone:
1-800-520-3011

Library | Contact

Distributors

For customers outside of the United States, please use one of our many distributors.

View Distributors

Payment Methods

We accept the following payment methods as well as pay-by-invoice.

MasterCard Visa PayPal
© 2023-2024 United States Biological - All Rights Reserved