Suitable for use in Flow Cytometry. Other applications not tested.

Flow Cytometry: Neat-1:5; 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher.

P3X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.

Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

MV4-11 acute monocyte leukemia cells.

Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).

Purified by Protein G affinity chromatography from tissue culture supernatant.

Recognizes human CD92. Specific for the C-­terminal variant of human CDw92 (isoform 3), also known as CTL1, a 70kD multi­pass membrane protein, expressed by monocytes, neutrophils, certain myeloid and T cell lines, and weakly by endothelial cells, fibroblasts and epithelial cells.