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C2443-81A Mouse Anti-CD98

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1,k
Clone Number
7H227
Grade
Affinity Purified
Applications
FC
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

Human CD98 is expressed on monocytes and activated cells. All cultured human cell lines tested express CD98. CD98 is thought to be an activation antigen.

Applications
Suitable for use in Flow Cytometry and Functional Assay. Other applications not tested.
Recommended Dilutions
Flow Cytometry: 1ug/ml. 80ul labels 5x10e5 Jurkat cells. Optimal dilutions to be determined by the researcher.
Flow Cytometry Protocol
1. Five x 10e5 cultured Jurkat human tumor cells were washed and incubated 45 minutes on ice with 80ul of C2443-81A at 1ug/ml. 2. Cells were washed twice and incubated with a goat x mouse IgG (FITC) (I1904-06F). 3. Cells were washed three times, fixed and analyzed by FACS. 4. Cells stained positive with a mean shift of 1.82 log10 fluorescent units when compared to a Mouse IgG1 negative control (I1904-78R) at a similar concentration.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Molt 13 T cell line.
Form
Supplied as a liquid in PBS, pH 7.5, 0.5mg/ml Gentamicin Sulfate.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes the heavy chain of 125kD human heterodimeric membrane glycoprotein CD98. Co-mitogenic with soluble CD2 and immobilized CD3 monoclonal antibodies.
References
1. Friedman, A.W., et al., Cell Immunol. 154: 253-263 (1994). 2. Leukocyte Typing V (S.F. Schlossman, et al., eds.) Oxford University Press, Oxford, p. 280-283 (1995).
USBio References
No references available
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