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You are here:Home » Antibodies » Abs to Cell Division Cycle (CDC) » Anti -cdc27 (Cell Division Cycle 27)

Anti -cdc27 (Cell Division Cycle 27)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IF
Human CDC27 (also called Cell division cycle protein 27 homolog, CDC27Hs and H-NUC) shares strong similarity with Saccharomyces cerevisiae protein Cdc27. This protein is a component of naphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of a cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis.
Catalog #C2550-11D
Cellular Localization Nuclear
Positive Controls Hela whole cell lysate
ApplicationsSuitable for use in ELISA, Western Blotting, Immunofluorescence. Other applications not tested.
Recommended DilutionELISA: 1:5000-1:40,000
Western Blotting: 1:500-1:2000. A 90kD band is seen (predicted MW 92kD)
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~2mg/ml
FormSupplied as a liquid in 0.15M sodium chloride and 0.02mM potassium phosphate [pH7.2], 0.01% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to a region between aa422 and 430 of human Cdc27.
SpecificityRecognizes human Cdc27. Expected to crossreact with chicken (100% identity with immunogen) due to sequence homology.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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