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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IP IH

Cyclin-dependent kinases (cdk) are the catalytic subunits of the cyclin/cdk complexes, which phosphorylate substrates on threonine/serine residues. Cdk2 interacts with cyclin D1, D3 and E, as well as cyclin A. Cyclin E/cdk2 kinase is active in G1- and S-phase of the cell cycle and is important for progression of cells from G1 to S. Cyclin A/cdk2 levels are maximal at the S/G2-phase transition and both are found associated with DNA in the initiation complex during replication. Rb is a substrate for cyclin E/cdk2 and cyclin A/cdk2 in vivo.
Catalog #	C2572-05B
Applications	Suitable for use in Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions	Immunoprecipitation: 10ul/mg; co-precipitates associated proteins
	Western Blot: 1ug/ml Incubate fot 2 hours at RT.
	Immunohistochemistry: 2-4ug/ml cryostat sections and paraffin embedded sections. For paraffin embedded sections, boil the sections in 1mM EDTA, pH 8, for 10-20 min followed by cooling at RT for 20 min.
	Optimal dilutions to be determined by the researcher.
Hybridoma	P3-NS1/AG4-1 (NS1) myeloma cells with spleen cells from Balb/c mice.
Positive Control	HeLa, 293, VA13, IMR90, Jurkat and WI-38 cells; thymus, tonsil or reactive lymph node.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2b
Clone No	5J207
Host	Mouse
Source	Human
Concentration	~0.1mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Purified recombinant human cdk2 protein.
Specificity	Recognizes human cyclin-dependent kinase-2 (cdk2) at ~33kD. Species Crosseactivity: mouse and rat. Does not cross-react with other members of the cdk family.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.