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You are here:Home » Antibodies » Abs to Protein Kinases » Anti -cdk 2(Cyclin-dependent Kinase 2)

Anti -cdk 2(Cyclin-dependent Kinase 2)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH
Cyclin-dependent kinases (cdk) are the catalytic subunits of the cyclin/cdk complexes, which phosphorylate substrates on threonine/serine residues. Cdk2 interacts with cyclin D1, D3 and E, as well as cyclin A. Cyclin E/cdk2 kinase is active in G1- and S-phase of the cell cycle and is important for progression of cells from G1 to S. Cyclin A/cdk2 levels are maximal at the S/G2-phase transition and both are found associated with DNA in the initiation complex during replication. Rb is a substrate for cyclin E/cdk2 and cyclin A/cdk2 in vivo.
Catalog #C2572-05B
ApplicationsSuitable for use in Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionsImmunoprecipitation: 10ul/mg; co-precipitates associated proteins
Western Blot: 1ug/ml Incubate fot 2 hours at RT.
Immunohistochemistry: 2-4ug/ml cryostat sections and paraffin embedded sections. For paraffin embedded sections, boil the sections in 1mM EDTA, pH 8, for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
HybridomaP3-NS1/AG4-1 (NS1) myeloma cells with spleen cells from Balb/c mice.
Positive ControlHeLa, 293, VA13, IMR90, Jurkat and WI-38 cells; thymus, tonsil or reactive lymph node.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No5J207
HostMouse
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenPurified recombinant human cdk2 protein.
SpecificityRecognizes human cyclin-dependent kinase-2 (cdk2) at ~33kD. Species Crosseactivity: mouse and rat. Does not cross-react with other members of the cdk family.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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