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Anti -CEACAM 3 (Carcinoembryonic antigen-related cell adhesion molecule 3, CD66d, CGM1)
Anti -CEACAM 3 (Carcinoembryonic antigen-related cell adhesion molecule 3, CD66d, CGM1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Sheep |
Affinity Purified |
B |
|
| Carcinoembryonic antigen-related cell adhesion molecule 3; also CD66d and CGM1) is a 31-34kD member of the CEACAM subfamily of the CEA family of proteins. It is expressed by neutrophils and serves as a microbial receptor for a wide range of microorganisms. Mature human CEACAM-3 is a 218 amino acid (aa) type I transmembrane glycoprotein. Its | | | extracellular domain (aa 35-155) shows one V-type Ig-like domain aa 35-142). There are two alternate splice forms. One shows a 40 aa substitution for the C-terminal 116 amino acids (aa 137-252), while a second shows a 31 aa substitution for the C-terminal 71 amino acids. No definitive rodent CEACAM-3 has been reported. | | | Catalog # | C2589-68B | | Applications | Suitable for use in Western blot. Other applications have not been tested. | | Recommended Dilutions | Western blot: 0. -0.2ug/ml with the The detection limit is ~1ng/lane under non-reducing and | | reducing conditions. | | Optimal dilutions should be determined by each laboratory for each application. | | Hybridoma | Produced in sheep immunized with purified, NS0-derived, recombinant human rhCEACAM-3; | | aa 33-155; Accession # P40198). Human CEACAM-3 specific IgG was purified by human CEACAM-3 affinity chromatography and then biotinylated. | | Storage and Stability | | | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Sheep | | Source | Human | | Concentration | ~ 0.05mg/ml | | Form | Supplied as a lyophilized powder in PBS with BSA. Reconstitute with 1ml sterile 0.1% BSA in TBS to a concentration of 0.05mg/ml. | | Purity | Purified by affinity chromatography. | | Immunogen | NS0-derived recombinant human rhCEACAM-3 extracellular domain isoform A. | | Specificity | Recognizes human CEACAM-3 extracellular domain. Cross-reactivity | | rhCEACAM-6 | 10%, and rhCEACAM-4 and rhCEACAM-5: 5%. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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