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You are here:Home » Antibodies » Abs to Cancer Markers » Anti -CEACAM 3 (Carcinoembryonic antigen-related cell adhesion molecule 3, CD66d, CGM1)

Anti -CEACAM 3 (Carcinoembryonic antigen-related cell adhesion molecule 3, CD66d, CGM1)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified B
Carcinoembryonic antigen-related cell adhesion molecule 3; also CD66d and CGM1) is a 31-34kD member of the CEACAM subfamily of the CEA family of proteins. It is expressed by neutrophils and serves as a microbial receptor for a wide range of microorganisms. Mature human CEACAM-3 is a 218 amino acid (aa) type I transmembrane glycoprotein. Its
extracellular domain (aa 35-155) shows one V-type Ig-like domain aa 35-142). There are two alternate splice forms. One shows a 40 aa substitution for the C-terminal 116 amino acids (aa 137-252), while a second shows a 31 aa substitution for the C-terminal 71 amino acids. No definitive rodent CEACAM-3 has been reported.
Catalog #C2589-68B
ApplicationsSuitable for use in Western blot. Other applications have not been tested.
Recommended DilutionsWestern blot: 0. -0.2ug/ml with the The detection limit is ~1ng/lane under non-reducing and
reducing conditions.
Optimal dilutions should be determined by each laboratory for each application.
HybridomaProduced in sheep immunized with purified, NS0-derived, recombinant human rhCEACAM-3;
aa 33-155; Accession # P40198). Human CEACAM-3 specific IgG was purified by human CEACAM-3 affinity chromatography and then biotinylated.
Storage and Stability
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceHuman
Concentration~ 0.05mg/ml
FormSupplied as a lyophilized powder in PBS with BSA. Reconstitute with 1ml sterile 0.1% BSA in TBS to a concentration of 0.05mg/ml.
PurityPurified by affinity chromatography.
ImmunogenNS0-derived recombinant human rhCEACAM-3 extracellular domain isoform A.
SpecificityRecognizes human CEACAM-3 extracellular domain. Cross-reactivity
rhCEACAM-610%, and rhCEACAM-4 and rhCEACAM-5: 5%.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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