Carcinoembryonic antigen-related cell adhesion molecule 3; also CD66d and CGM1) is a 31-34kD member of the CEACAM subfamily of the CEA family of proteins. It is expressed by neutrophils and serves as a microbial receptor for a wide range of microorganisms. Mature human CEACAM-3 is a 218 amino acid (aa) type I transmembrane glycoprotein. Its extracellular domain (aa 35-155) shows one V-type Ig-like domain aa 35-142). There are two alternate splice forms. One shows a 40 aa substitution for the C-terminal 116 amino acids (aa 137-252), while a second shows a 31 aa substitution for the C-terminal 71 amino acids. No definitive rodent CEACAM-3 has been reported.
Applications
Suitable for use in Western blot. Other applications have not been tested.
Recommended Dilutions
Western blot: 0. -0.2ug/ml with the The detection limit is ~1ng/lane under non-reducing and reducing conditions. Optimal dilutions should be determined by each laboratory for each application.
Hybridoma
Produced in sheep immunized with purified, NS0-derived, recombinant human rhCEACAM-3; aa 33-155; Accession # P40198). Human CEACAM-3 specific IgG was purified by human CEACAM-3 affinity chromatography and then biotinylated.
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
NS0-derived recombinant human rhCEACAM-3 extracellular domain isoform A.
Form
Supplied as a lyophilized powder in PBS with BSA. Reconstitute with 1ml sterile 0.1% BSA in TBS to a concentration of 0.05mg/ml.
Purity
Purified by affinity chromatography.
Specificity
Recognizes human CEACAM-3 extracellular domain. Cross-reactivity: rhCEACAM-1:15%, |rhCEACAM-6: 10%, and rhCEACAM-4 and rhCEACAM-5: 5%.
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