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You are here:Home » Antibodies » Abs to Nuclear Proteins » Anti -Centaurin, beta 1 (CENTB1, ACAP1, ACAP1, KIAA0050, KIAA0050 gene product, Arf GAP with coiled coil, ANK repeat and PH domains 1)

Anti -Centaurin, beta 1 (CENTB1, ACAP1, ACAP1, KIAA0050, KIAA0050 gene product, Arf GAP
with coiled coil, ANK repeat and PH domains 1)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
Centaurin beta 1 and Centaurin beta 2 are recruited to platelet-derived growth factor-induced dorsal membrane ruffles in NIH 3T3 mouse fibroblasts, and overexpression inhibited ruffle formation. In vitro, Centaurin beta 1 and Centaurin beta 2 preferred ARF6 as substrate rather than ARF1 or ARF5, and the GAP activity of both Centaurins was dependent upon phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). The isolated PH domain of mouse Centaurin beta 2 exhibits moderate affinity for PtdIns(3,5)P2, but it does not bind any other phosphoinositides tested, including PtdIns(4,5)P2. Mutation of a highly conserved arginine in both Centaurins result in lack of ARF-GAP activity. Overexpression in HeLa cells of either Centaurin blocks the formation of ARF6-dependent protrusions. Both Centaurins are also recruited to peripheral, tubular membranes, where activation of ARF6 occurs and allows membrane recycling back to the plasma membrane.
Catalog #C2617-02C1
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:32000
Western Blot: 0.5-2ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostGoat
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in Tris-saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide of ACAP1.
SpecificityRecognizes human ACAP1 at ~80-90kD in Jurkat lysates (predicted MW of 85kDa according to NP_055531). Species Crossreactivity: Mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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