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You are here:Home » Molecular Biology » MB-Enzymes, Glycosidase » Chitinase, Trichoderma viride (Chitodextrinase, N-acetyl- -glucosaminidase)

Chitinase, Trichoderma viride (Chitodextrinase, N-acetyl- -glucosaminidase)

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Specifications

The chitinolytic enzymes from T. viride are a mixture of extracellular chitinolytic enzymes, which exhibit exo and endochitinase activities including N-acetyl- -glucosaminidase and chitobiosidase.
Catalog #C4154-20A
Chitinases are digestive enzymes that break down glycosidic bonds in chitin. Because chitin composes the cell walls of fungi and exoskeletal elements of some animals (including worms and arthropods), chitinases are generally found in organisms that either need to reshape their own chitin or to dissolve and digest the chitin of fungi or animals.
SolubilitySoluble in phosphate buffer, pH 6.1, (1mg/ml), yielding a clear to faint hazy, tan solution
Chitinase Activity 600 units/g solid
Chitobiosidase Activity:
As reported
Nagase Activity:
As reported
Optimum pH: 7.8~8.6
pH Stability (30°C, 1 hour) 6.6~9.0
Temperature Stability 45°C (10 min.)
Chitinase Unit Definition:
One unit will liberate 1mg of N-acetyl-D-glucosamine from chitin per hour at pH 6.0 at 25°C in a 2 hour assay.
Chitobiosidase Unit DefinitionN-Acetyl-b-glucosaminidase
[Chitobiosidase]: One unit will release 1.0 mg of 4-nitrophenol from 4-nitrophenyl N-Acetyl-b-Dglucosaminide [4-nitrophenyl N,N'-diacetyl -b--Dchitobioside] per minute at pH 6.1 at 40°C.
AssayReaction mixture is composed of 30mg of N-acetyl chitosan, 2ml of 0.1M Mcllvaine buffer (pH 6.8) and 1ml of enzyme solution. After incubation at 37°C for 60 minutes, the reaction of the enzyme is terminated by heating at 100°C for 5 minutes and the amount of reducing sugar is determined by the method of Schales and Schales, using N-acetyl glucosamine as a standard.
Storage & StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute with sterile PBS. Store at -20°C. Reconstituted product is stable for 5 weeks at 4°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap
Molecular Weight110-115kD
SourceTrichoderma viride
FormSupplied as a lyophilized powder.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesEC=3.2.1.14; Chitodextrinase; N-acetyl- -glucosaminidase


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