Suitable for use in Immunofluorescence of cell culture and frozen tissue sections. Detection of chlamydia in clinical specimen after fixation with methanol/acetone (1:1). Other applications have not been tested.

Immunofluorescence (IC): 1:10 Immunohistochemistry: Frozen Optimal dilutions to be determined by the researcher.

Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Chlamydia antigen

Supplied as a lyophilized powder from PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O.

Purified by Protein A affinity chromatography.

Recognizes C. trachomatis, C. psittaci. Recognizes a species-specific epitope on the 40kD major outer membrane protein of C. trachomatis with a strong fluorescence of elementary bodies (EBs), reticulate bodies (Rbs) and cytoplasmic inclusions of B-complex serotypes. C-complex serotypes show a weak reaction.