| Catalog # | C5069-68 |
| Intended Use | For the quantitative determination of free beta subunit of human chorionic gonadotropin (free Beta-hCG) concentration in human serum. |
| Standard Size | 96 wells |
| Range | 0-50ng/ml |
| Sensitivity | 2.5ng/ml |
| Crossreactivity | This kit has been validated for use with human serum samples only. |
| Principle of the Test | The free beta-hCG ELISA test is based on the principle of a solid phase enzyme-linked immunosorbent assay. The assay system utilizes a unique monoclonal antibody directed against a distinct antigenic determinant on the free beta-hCG. Mouse monoclonal anti-free-beta-hCG antibody is used for solid phase immobilization (on the microtiter wells). A goat anti-whole hCG antibody is in the antibody-enzyme (horseradish peroxidase) conjugate solution. The test sample is allowed to react sequentially with the two antibodies, resulting in the free beta-hCG molecules being sandwiched between the solid phase and enzyme-linked antibodies. After two separate 30 minute incubations at 37°C, the wells are washed with water to remove unbound labeled antibodies. A solution of TMB Reagent is added and incubated for 20 minutes, resulting in the development of a blue color. The color development is stopped with the addition of Stop Solution changing the color to yellow. The concentration of beta-hCG is directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450 nm. |
| Kit Components | C5069-68A: Microtiter Plate, 1x96wells (in strips). Coated with murine monoclonal anti-free beta-hCG antibody |
| C5069-68B: Calibrator 0ng/ml, 1x1vial. Lyophilized. |
| C5069-68C: Calibrator 2.5ng/ml, 1x1vial. Lyophilized. |
| C5069-68D: Calibrator 5ng/ml, 1x1vial. Lyophilized. |
| C5069-68E: Calibrator 10ng/ml, 1x1vial. Lyophilized. |
| C5069-68F: Calibrator 25ng/ml, 1x1vial. Lyophilized. |
| C5069-68G: Calibrator 50ng/ml, 1x1vial. Lyophilized. |
| C5069-68H: Sample Diluent, 1x13ml |
| C5069-68J: Pab (HRP), 1x18ml |
| C5069-68K: TMB Solution, 1x11ml |
| C5069-68L: Stop Solution, 1x11ml |
| Storage and Stability | Store kit components at 4°C. Unopened kit components are stable for 6 months at 4°C. Reconstituted standards are stable for up to 30 days when stored sealed at 4°C. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
