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C5828-01A Rat Anti-Clara Cell Protein (CC16, CC10, Uteroglobin, Urinary Protein 1, Clara Cell Secretory Protein)

Specifications
Clone Type
Monoclonal
Host
Rat
Source
Human
Swiss Prot
P11684
Isotype
IgG1
Clone Number
7H197
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Hu
Accession #
NP_003348
Shipping Temp
Blue Ice
Storage Temp
-20°C

Uteroglobin, previously called CCPBP, CC10 or blastokinin, is a small secreted protein of the secretoglobin (family 1A, member 1). Uteroglobin is expressed by Clara cells, the non-ciliated, non-mucous secretory cells predominant in lung bronchioles, and by other epithelia that communicate with the external environment. Uteroglobin binds to phospholipids and to several cell types, inhibiting inflammation, migration and invasion. It has been reported to bind the lipocalin-1 receptor. The mature 70aa protein forms a disulfide-linked heterodimer of 16kD.

Applications
Suitable for use in Immunohistochemistry, Western Blot and ELISA. Other applications not tested.
Recommended Dilutions
Immunohistochemistry: 8-25ug/ml using immersion fixed paraffin-embedded sections of human lung subjected to antigen retrieval reagent-basic Western Blot: 1ug/ml using recombinant human Uteroglobin Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to Glu22-Asn91 from human Clara Cell Protein, expressed in E. coli.
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity
Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Specificity
Recognizes human Clara Cell Protein.
Conjugates
Pricing
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