| Cofilin is a small phosphoinositide-sensitive, actin-binding protein capable of depolymerizing actin filaments in vitro. Under certain conditions, it fragments the filaments and accelerates actin subunit dissociation from their |
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| Catalog # | C7506-15A |
| Applications | Suitable for use in Immunofluorescence and Western Blot. Other applications not tested. |
| Recommended Dilution | Western Blot: 1:1000. Detects a band at approximately 19kDa. Use whole extracts of human A-431 epidermoid carcinoma, rat PC-12 pheochromocytoma, and dog MDCK kidney cells, or purified recombinant chicken muscle cofilin. |
| Immunofluorescence: 1:1000 using NIH-NT3 fibroblasts |
| Optimal dilutions to be determined by the researcher. |
| Cellular Localization | Cytoplasmic and Nuclear |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | Not determined |
| Form | Supplied as a liquid in PBS, pH 7.4, containing sodium azide |
| Purity | Purified |
| Immunogen | Synthetic peptide corresponding to amino acids 154-166 of human cofilin with N-terminal cysteine added, conjugated to maleimide-activated keyhole limpet hemocyanin (KLH). The corresponding immunizing sequence is identical in pig and rat non-muscle cofilin and differs by three amino acids from that of human and chicken muscle cofilin. |
| Specificity | Recognizes human Cofilin. Species Crossreactivity: mouse, rat, chicken, canine and porcine. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
| Alternate names | 18kD phosphoprotein, CFL1, Cofilin 1 non muscle, Cofilin non muscle isoform, p18 |
