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You are here:Home » Antibodies » Abs to Actin Related Proteins » Anti -Cofilin

Anti -Cofilin

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Purified B IF
Cofilin is a small phosphoinositide-sensitive, actin-binding protein capable of depolymerizing actin filaments in vitro. Under certain conditions, it fragments the filaments and accelerates actin subunit dissociation from their
Catalog #C7506-15A
ApplicationsSuitable for use in Immunofluorescence and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000. Detects a band at approximately 19kDa. Use whole extracts of human A-431 epidermoid carcinoma, rat PC-12 pheochromocytoma, and dog MDCK kidney cells, or purified recombinant chicken muscle cofilin.
Immunofluorescence: 1:1000 using NIH-NT3 fibroblasts
Optimal dilutions to be determined by the researcher.
Cellular Localization Cytoplasmic and Nuclear
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in PBS, pH 7.4, containing sodium azide
PurityPurified
ImmunogenSynthetic peptide corresponding to amino acids 154-166 of human cofilin with N-terminal cysteine added, conjugated to maleimide-activated keyhole limpet hemocyanin (KLH). The corresponding immunizing sequence is identical in pig and rat non-muscle cofilin and differs by three amino acids from that of human and chicken muscle cofilin.
SpecificityRecognizes human Cofilin. Species Crossreactivity: mouse, rat, chicken, canine and porcine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate names18kD phosphoprotein, CFL1, Cofilin 1 non muscle, Cofilin non muscle isoform, p18


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